ISSN:
1573-5001
Keywords:
2D heteronuclear NMR
;
Isotope labeling
;
Coupling constants
;
Antamanide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A simple heteronuclear relayed E.COSY pulse sequence with a minimum number of pulses is proposed for the quantitative determination of heteronuclear three-bond J-coupling constants in uniformly 13C-enriched polypeptide samples. Numerous heteronuclear three-bond coupling constants, including $${}^3{\text{J}}_{{\text{H}}^{\text{N}} {\text{C}}'} $$ , $${}^3{\text{J}}_{{\text{H}}^{\text{N}} {\text{C}}^\beta } $$ , $${}^3{\text{J}}_{{\text{H}}^\beta {\text{C}}'} $$ , and $${}^3{\text{J}}_{{\text{H}}^\alpha {\text{C}}^\gamma } $$ , can be determined for each residue from a single heteronuclear relayed E.COSY spectrum. Couplings relevant for stereospecific assignments as well as for the determination of dihedral angles in the amino acid backbone and in side chains are obtained. The method is demonstrated on the uniformly 13C-enriched decapeptide antamanide (-Val1-Pro2-Pro3-Ala4-Phe5-Phe6-Pro7-Pro8-Phe9-Phe10-).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00417495
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