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  • Escherichia coli  (2)
  • Copy number  (1)
  • Entwicklungsländer  (1)
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  • 1
    ISSN: 1436-6215
    Keywords: protein-energymalnutrition ; vitaminE status ; children ; Sudan ; developingcountries ; Protein-Energie-Mangelernährung ; Vitamin-E-Status ; Kinder ; Sudan ; Entwicklungsländer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Das Gesamttocopherol und alpha-Tocopherol wurden im Plasma von Kindern mit schwerer Mangelernährung (14 Maramus; 11 marantischer kwashiorkor; 5 kwashiorkor) bestimmt und auf die Gesamtlipide des Plasmas und auf die verschiedenen Plasmalipidklassen bezogen. Wenn die reinen Plasmakonzentrationen als ein Index des Vitamin-E-Status genommen würden, so wären 5 Kinder mit Marasmus, 5 Kinder mit marantischem kwashiorkor und 2 Kinder mit kwashiorkor als defizitär (〈500 μg/dl) anzusehen. Werden jedoch Gesamttocopherol und alpha-Tocopherol auf die Gesamtlipide bezogen, so zeigten alle mangelernährten Kinder — bis auf eines — Werte, die innerhalb der Grenzen lagen, die für gesunde amerikanische Kinder gefunden wurden. Die Studie zeigt, daß niedrige Tocopherol-Lipid-Verhältnisse kein konstantes Merkmal bei schwer mangelernährten Kindern sind.
    Notes: Summary Total tocopherols and alpha-tocopherols were estimated in the plasma of children with severe malnutrition (14 marasmus; 11 marasmic kwashiorkor; five kwashiorkor) and related to the total plasma lipids and different plasma lipid classes. If the mere plasma concentrations were taken as an index of the vitamin E status, five children with marasmus, five children with marasmic kwashiorkor, and two children with kwashiorkor would have been regarded as deficient (〈500 μg/dl). However, if total tocopherols and alpha-tocopherols were related to the total plasma lipids, all malnourished children — except one — showed values within the limits found in healthy American children. The study shows that low tocopherol/lipid ratios are not a constant feature in severely malnourished children.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 211 (1988), S. 138-142 
    ISSN: 1617-4623
    Keywords: Escherichia coli ; DnaA ; Replication initiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Overexpression of DnaA protein from a multicopy plasmid accompanied by a shift to 42°C causes initiation of one extra round of replication in a dnaA + strain grown in glycerol minimal medium. This extra round of replication does not lead to an extra cell division, such that cells contain twice the normal number of chromosomes.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 213 (1988), S. 379-387 
    ISSN: 1617-4623
    Keywords: Stringent control ; RNA polymerase ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Previous studies on two Escherichia coli rpoB mutants, carrying single amino acid substitutions at approximate amino acid positions 736 and 906 in the β subunit, showed that these alterations in the RNA polymerase resulted in an apparent reduced response to valine-induced amino acid starvation in vivo and prevented ppGpp-mediated inhibition of transcriptional initiation at stable RNA promoters in vitro. These observations suggested that the mutations had altered either the ppGpp binding site or the promoter selectivity of the enzyme. The in vivo analysis presented here indicates that these mutants encode an RNA polymerase that responds normally to changes in the level of ppGpp; their apparent relaxedness is due to a reduced accumulation of ppGpp during isoleucine starvation. Thus, there is no indication that the mutations have altered ppGpp binding sites. These observations and the difference between in vitro and in vivo results can be explained by the assumption that the mutations produce an extended ppGpp-dependent pausing of RNA polymerase during the transcription of unstable RNA. Comparison of the vivo and in vitro effects of ppGpp on rrn transcription further suggests that these reflect different phenomena, although in both cases ppGpp inhibits rrn transcription.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 203 (1986), S. 143-149 
    ISSN: 1617-4623
    Keywords: Plasmid ; pBR322 ; Copy number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The concentration of plasmid pBR322, of its replication inhibitor, RNAI, and preprimer, RNAII, were observed in E. coli as functions of the bacterial growth rate. At growth rates between 0.6 and 2.5 doubling/h, the copy number (number of plasmids per genome equivalent of chromosomal DNA) decreased from 32 to 15, the number of plasmids per cell increased fro, 39 to 55, and the plasmid concentration decreased from 4.6 to 1.1x1010 plasmids per OD460 unit of cell mass. The concentrations of RNAI and RNAII also decreased with incrasing growth rate, but differently, such that their ratio, RNAI/RNAII, increased. In glycerol minimal medium both RNAI and RNAII had the same halflife, 0.55 min, and were synthesized at a ratio of about 3 RNAI transcripts per every RNAII transcript. These results were interpreted on the basis of the negative control model and suggest that the activities of the RNAI and RNAII promoters, and the efficiency with which RNAI inhibits plasmid replication, are controlled by the growth rate.
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