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  • Biochemistry and Biotechnology  (3)
  • Constant volume Varigrad  (2)
  • Wiley-Blackwell  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Distance indices in a comparison between the A, D, I and R genomes of the Triticeae tribe (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1295-1300 
    Details
    ISSN: 0173-0835
    Keywords: Triticum ; Secale ; Hordeum ; Two-dimensional polyacrylamide gel electrophoresis ; Phylogenetic relationships ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Two-dimensional gel electrophoresis of shoot proteins was used to study the relationships between Triticum, Secal, and Hordeum. A high level of polymorphism was found among the 1275 spots scored: only 198 spots were found common to all. But, under the hypothesis that only allelic variations were observed, the mean number of alleles per locus was only two. Phenograms were built from different distance indices. All of them showed Triticum genomes A and D close to each other, Hordeum, far from the Triticum cluster, and Secale at an intermediate position. A discussion on the use of various distance indices is presented.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601212
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  • 2
    Electronic Resource
    Electronic Resource
    The Varigrad and similar apparatus in gradient liquid chromatography: A systematic computational approach. Part 1: Theoretical development (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 507-515 
    Details
    ISSN: 0935-6304
    Keywords: Constant volume Varigrad ; Hydrostatic Varigrad ; Incremental method of gradient elution ; Modified incremental method, advantages of Poisson distribution ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Different methods for producing a pre-assigned gradient in liquid chromatography are presented. A systematic approach to the calculations involved is given. The derived equations are mostly Poisson and Poisson summation distributions which are tabulated in the literature. The incremental method of gradient elution developed by Scott has been modified. In the modified apparatus two mixing chambers are used instead of one. This leads to an appreciable decrease in the number of reservoirs needed for the same precision in fitting a desired gradient. The application of the derived equations, together with other Varigrad modifications, will be given in Part 2 of this paper (to be published shortly in this Journal).
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240031005
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  • 3
    Electronic Resource
    Electronic Resource
    The varigrad and similar apparatus in gradient liquid chromatography: A systematic computational approach. Part 2: Applications (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 575-582 
    Details
    ISSN: 0935-6304
    Keywords: Numerical examples ; Constant volume Varigrad ; Hydrostatic Varigrad ; Incremental method of gradient elution ; Modified incremental method ; Non-homogeneous Varigrad ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Part 2 deals with the application of the equations derived in Part 1 to the systematic calculations of the constant volume and hydrostatic Varigrads. Examples are given which illustrate the advantage of using the modified incremental method of gradient elution. The non-homogeneous Varigrad, where mixing chambers may vary in volume, is also treated.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240031106
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and propagation of new methanol-utilizing microorganisms (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 25 (1983), S. 863-865 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260250319
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  • 5
    Electronic Resource
    Electronic Resource
    Single-cell protein from methanol with Enterobacter aerogenes (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 29 (1987), S. 355-357 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An identifiedEnterobacter aerogenesutilizing methanol as a sole carbon source was studied for the optimization of biomass production and the reduction of its nucleic acid content. Results indicated that the highest yield and conversion were obtained at 0.5% methanol. The addition of seawater as a source of trace elements has an adverse effect. However, the addition of urea as source of nitrogen enhanced the growth of E. aerogenes. Heat shock at 60°C for 1 min followed by incubation at 50°C for 2 h caused 72.6% reduction in the nucleic acid.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260290310
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