ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Epistatic interaction among loci controlling the palmitic and the stearic acid levels in the seed oil of sunflower (2000)

Pérez-Vich, B. ; Garcés, R. ; Fernández-Martínez, J. M.

Springer

Theoretical and applied genetics 100 (2000), S. 105-111

add to mindlist on the mindlist

Details

ISSN: 1432-2242

Keywords: Key words Sunflower ; Helianthus annuus ; High palmitic acid ; High stearic acid ; Epistatic interaction ; Inheritance ; Oil quality

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Two sunflower (Helianthus annuus L.) mutants with high concentrations of saturated fatty acids in their seed oil have been identified and studied extensively. The mutant line CAS-5 has high concentrations of palmitic acid (C16:0) (〉25% compared with 7% in standard sunflower seed oil) and low-C18:0 values (3%). CAS-3 is characterized by its high levels of stearic acid (C18:0) (〉22% compared with 4% in standard sunflower seed oil) and a low-C16:0 content (5%). CAS-5 also possesses elevated levels of palmitoleic acid (C16:1) (〉5%), which is absent in standard sunflower seed oil. The objective of this study was to determine the relationships between the loci controlling the high-C16:0 and the high-C18:0 traits in these mutants. Plants of both mutants were reciprocally crossed. Gas chromatographic analyses of fatty acids from the seed oil of F1, F2, F3 and the BC1F1 to CAS-5 generations indicated that the loci controlling the high-C16:0 trait exerted an epistatic effect over the loci responsible for the high-C18:0 character. As a result, the phenotypic combination containing both the high-C16:0 levels of CAS-5 and the high-C18:0 levels of CAS-3 was not possible. However, phenotypes with a saturated fatty acid content of 44% (34.5% C16:0+9.5% C18:0) were identified in the F3 generation. These are the highest saturated (C16:0 and C18:0) levels reported so far in sunflower seed oil. When F3 C16:0 segregating generations in both a high- and a low-C18:0 background were compared, the high-C16:1 levels were not expressed as expected in the high-C18:0 background (CAS-3 background). In this case, the C16:1 content decreased to values below 1.5%, compared with 〉5% in a low-C18:0 background. As the stearoyl-ACP desaturase has been reported to catalyze the desaturation from C16:0-ACP to C16:1-ACP, these results suggested that a decrease in its activity was involved in the accumulation of C18:0 in the high-C18:0 mutant CAS-3.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050015

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

The γ-butyrolactone ring puckering (1989)

Galache, M. P. ; Garcés, P. ; Esteban, A. L. ; [et al.]

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 27 (1989), S. 44-49

add to mindlist on the mindlist

Details

ISSN: 0749-1581

Keywords: γ-Butyrolactone ; 1H NMR analysis ; Conformational analysis ; Molecular mechanics ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The 60 and 100 MHz 1H NMR spectra of γ-butyrolactone were analysed in detail. The best set of coupling values (Hz ± 0.03) is 3JABc = 7.86, 3JABt = 6.27, 3JBCc = 9.52, 3JBCt = 6.86, 4JACc + 4JACt = -0.18, 2JA - 2JB = 3.89 and 2JB - 2JC = 4.86 (A, B and C refer to α, β and γ protons, with respect to the ring oxygen, and c and t mean the same and opposite side of the ring, respectively). The 3J values support the conclusion that the molecule is interconverting between two equivalent conformations of near envelope type with the β-carbon out of the mean plane defined by the fragment C—O—CO—C.

Additional Material: 3 Ill.