ISSN:
1432-0878
Keywords:
Monoamine fluorescence
;
Microfluorometry
;
Computer-assisted correction
;
Hypothalamus
;
Rat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary In a circumscribed area of the preoptic periventricular nucleus of a male rat, formaldehyde-induced monoamine fluorophores modified by treatment with HCl vapors were investigated microfluorometrically (measurement of excitation peak ratio 370∶320 nm) in all fluorescent terminals and preterminals. Microfluorometric recordings of an individual fluorescent structure were performed without UV irradiation of neighboring fluorophores. Recorded data were sampled and corrected by a microcomputer (WangPCS II). 19 neuronal processes (axons) contained noradrenaline fluorophores; 11 contained dopamine fluorophores; 6 exhibited uncharacteristic excitation peak ratios; and in 9 recordings technical problems did not allow identification of the fluorophore content.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00237008
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