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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 11 (1998), S. 131-139 
    ISSN: 1432-2145
    Keywords: Key words Calmodulin ; mRNA ; Calcium ; Pollen tube ; Agapanthus umbellatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Pollen tube growth is a vital process for angiosperm fertilisation and is dependent on the presence of a tip-focused gradient of cytosolic free calcium ([Ca2+]c). In order to clarify some of the target molecules which convey the Ca2+ signal information, we investigated calmodulin distribution during tube growth. Fluorescently labelled calmodulin was pressure microinjected into pollen tubes and its distribution monitored by confocal microscopy. Calmodulin distributes evenly throughout the cell, but some of its binding sites form a V-shaped collar behind the apical region. This specific association dissipates upon growth arrest, and suggests an interaction of calmodulin with cytoskeletal-bound target proteins. The distribution of calmodulin mRNA was also analysed by microinjection of fluorescently labelled mRNA. No specific pattern was observed, with an even localisation in the body of tube and a lower concentration in the cell apex. Studies with localised application of inhibitors/activators indicate that calmodulin plays a crucial role in tip elongation but does not direct tube orientation.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-07-17
    Description: Diverse space-related applications have been proposed for microscopic and sub-microscopic structures, mechanisms, and 'organisms'. To govern their functions, many of these tiny systems will require even smaller, nanometer-scale programmable computers, i.e. 'nanocomputers' on-board. This paper provides an overview of the results of a nearly two-year study of the technologies and designs that presently are in development for electronic nanocomputers. Strengths and weaknesses of the various technologies and designs are discussed, as well as promising directions for remedying some of the present research issues in this area. The presentation is a synopsis of a longer MITRE review article on the same subject.
    Keywords: Computer Operations and Hardware
    Type: Proceedings of the International Conference on Integrated Micro/Nanotechnology for Space Applications; NASA-CR-201963
    Format: text
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