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  • Chemistry  (9)
  • dihaploid  (3)
  • Compartmentation  (1)
  • 1975-1979  (13)
  • 1978  (13)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 116 (1978), S. 275-278 
    ISSN: 1432-072X
    Keywords: Yeast ; Polyphosphate ; Compartmentation ; Vacuole ; Cell wall
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Virtually all of the polyphosphate (PP) present in yeast protoplasts can be recovered in a crude particulate fraction if polybase-induced lysis is used for disrupting the protoplasts. This fraction contains most of the vacuoles, mitochondria and nuclei. Upon the purification of vacuoles the PP is enriched to the same extent as are the vacuolar markers. The amount of PP per vacuole is comparable to the amount of PP per protoplast. The possibility that PP is located in the cell wall is also considered. In the course of the incubation necessary for preparing protoplasts, 20% of the cellular PP is broken down. As this loss of PP occurs to the same extent in the absence of cell wall degrading enzymes, it is inferred that internal PP is metabolically degraded, no PP being located in the cell walls. It is concluded that in Saccharomyces cerevisiae most if not all of the PP is located in the vacuoles, at least under the growth conditions used.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; incompatibility ; dihaploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Investigations of the genetics of self-compatibility and self-incompatibility in dihaploids and diploid derivatives from cv. Gineke revealed the presence of S 1, S2 and S 3 at the S-locus of Gineke and in addition an S 1-allele on a translocation. By means of a complete tester set involving the S-alleles S 1, S2 and S 3 (all from Gineke) and S 4 (from Black 4495) it was demonstrated that some Gineke dihaploids were compatible with all six testers. This indicated a fourth S-allele in Gineke, which differs from those in the tester series and was therefore assigned S 5. Additional evidence was obtained from an analysis of F1's from crosses of two S 5-bearing dihaploids and one of the testers. So the S-genotype of cv. Gineke was identified as S 1S2S3S5/S1, the second S 1 being the S-allele on a translocated fragment.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5060
    Keywords: Potato ; dihaploid ; marker genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the first inbred generation (I1) of cv. Black 4495 a dark green, slowly growing mutant (coded ds) was found, whereas the I1 of its self-compatible dihaploid, B16, comprised this ds mutant and in addition the mutants virescens (v) and yellow margin (ym). The occurrence of ds and ym might trace back to diploid S. phureja, one of the ancestors of Black 4495. No lethal mutants were observed in I1 of B16. Analysis of I1 of cv. Gineke revealed a simplex condition for virescence and either duplex or triplex heterozygosity for one lethal gene. On the other hand, the I1 of its dihaploid, G254, segregated for virescence and for three different lethal genes. It is shown that both in B16 and in G254 homozygosity of an S-bearing translocation causes early death of embryo and endosperm, thus preventing seed development. From this study it appeared that the three lethal genes from G254 affect germination rate of the seeds. The genotypes at 11 loci of B16 and G254 are presented.
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  • 4
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; self-compatibility ; dihaploid ; linkage ; virescens ; translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three dihaploids of Solanum tuberosum (two self-compatible, one self-incompatible) were found to be heterozygous for a monogenic recessive virescent mutant. Intercrossing resulted in the expected 3 : 1 ratio only in crosses involving one self-compatible and one self-incompatible parent. Self-compatible x self-compatible matings produced F1's in which 6:1 was found. The same ratio was observed in the self progeny of the two self-compatible dihaploids. This significant deviation could be explained by assuming linkage (25% crossing-over) between v and an S-bearing translocation. This translocation causes self-compatibility in the dihaploids used and early lethality when homozygous.
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 9 (1978), S. 406-413 
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Form and Mechanisms of Cracks and Fractures Caused by Simultaneous Corrosive and Dynamic Stress under Various FrequenciesDistinction of crack nucleation and crack propagation under variable frequencies and electrolytic corrosion. Relationship between crack nucleation and frequency due to differing sliding mechanisms. Crack propagation under decreasing frequencies causes transition from corrosion fatigue to cyclic stress corrosion cracking and finally stress corrosion cracking can be observed. Coordination of that transition with the increasing influence of the KISCC-value as determinant of the crack propagation.The influence of frequency on high temperature materals in hot gas atmosphere is mainly determined by creep mechanisms during top-load phase and the gas-metal-reactions. Accordingly the life time increases with growing load frequency.
    Notes: Anrißphase und Phase des Rißfortschritts bei der Betrachtung der Frequenzabhängigkeit unter elektrolytischer Korrosion. Abhängigkeit der Anrißphase von der Frequenz infolge unterschiedlicher Gleitmechanismen. Beim Rißfortschritt erfolgt mit sinkender Frequenz Übergang von Schwingungsrißkorrosion zu zyklischer Spannungsrißkorrosion und schließlich zu Spannungsrißkorrosion. Zuordnung dieses Übergangs zum zunehmenden Einfluß des KISCC-Wertes als Bestimmungsgröße für den Bruchvorgang.Frequenzabhängigkeit bei Hochtemperaturwerkstoffen in Heißgasatmosphäre im wesentlichen bestimmt durch Kriechvorgänge bei Einwirkung der Oberlast und zeitabhängige Gas-Metall-Reaktionen. Entsprechend verlängern sich die Standzeiten mit zunehmender Belastungsfrequenz.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 1 (1978), S. 97-97 
    ISSN: 0935-6304
    Keywords: Liquid Chromatography ; Structure and relation of polyhalogenated aromatics ; discussed ; Silica gel/aluminium oxide compared ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Organic radicals were produced in the tensides C16H33(OCH2CH2)21OH and C14H29(OCH2CH2)3-SO3Na in aqueous solutions using a short pulse of high energy electrons. The radicals were formed by OH attack on the (OCH2CH2)x-parts of the tensides. The decay of the 250nm absorption of the radicals was recorded at different initial radical concentrations and tenside concentrations. Several radicals could be produced in one micelle. Radicals formed in the same micelle decay within microseconds or faster. The half life time τ1 in a micelle carrying two radicals is 2,0·10-6 s for C16H33(OCH2CH2)21 OH and less than 6·10-7 s for C14H29(OCH2CH2)3SO3Na. A model for intramicellar radical-radical reactions is proposed according to which the rate is faster in tensides of high critical micelle concentration. Single radicals in micelles of C16H33(OCH2CH2)21OH can deactivate each other without leaving the micelles. This intermicellar reaction is discussed in terms of the rate of diffusion-controlled micelle-micelle encounters, an encounter time of 7·10-8s, and the above time τ1 for intramicellar reaction. The observed rate constant 2k of intermicellar reaction is 3,5·106 mol-1·l·s-1. At low tenside concentrations, the bimolecular rate constant increases since more single tenside radicals are present in solution. They react rapidly (ca. 108 mol-1·l·s-1) with radicals in micelles. Single radicals in C14H33(OCH2CH2)3-SO3Na micelles cannot directly react with each other because of the Coulombic repulsion between two anionic micelles. Reaction occurs after the exit of a tenside radical from its micelle, the rate of which depends on the micellar equilibrium Mn⇄Mn-1+M (M: tenside molecule; n: agglomeration number of micelle). A single radical in solution reacts with a single radical in a micelle with 2k = 1,0·108 mol-1·l·s-1 and with another single radical in solution with 4,0·107 mol-1·l·s-1.γ-Irradiation of both tensides in aqueous solution leads to slight increases in viscosity, followed by turbidity beyond the “gel dose” and phase separation. These effects are explained in terms of crosslinking of tenside molecules. Formation of a large network requires bridges between all participating tenside molecules (not only bridges between micelles).
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 50 (1978), S. 693-695 
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 320 (1978), S. 493-496 
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Preparation of Micro-Packed Columns for Gas-Solid Chromatography with Graphitized Thermal Carbon Black
    Additional Material: 3 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Biopolymers 17 (1978), S. 1973-1986 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The backbone and side-chain conformations of the bicyclic octapeptide α-amanitin indimethylsulfoxide (DMSO) solution have ben deduced from analysis of the nmr spectrl parameters and conformational energy calculations. Several ambiguities in the nmr spectral assignments were resolved following a comparison with the recently published conformation of β-amanitin in the crystalline state. The peptide proton exchange and temperature coefficient data demonstrate strong intramolecular hyfrogen bonds for the GLY5 and Cys8 peptide protons. The vicinal proton coupling constants are consistent with the cyclic octapeptide udergoing chain reversl at the Ile6-Gly7 abd the Hyp2-Hyi3 dipeptide segments. The upfield shifts of the glycine and isoleucine protons demonstrate the folding of the indole ring of the Trp4-Cys8 brifge towards the Gly5-Ile6-Gly7 half of the Ile-amanitin molecule. The structure af α-amanitin in DMSO is defined by the (φψ) backbone rotation angles Trp4(-90, -60), Gly5 (+120, -120), Ile6(-6, +120), Gly7 (+45, +60), Cys8(-120, -60), Asn1 (+175, -175), Hyp2 (-160, -45), and Hyi3 (-90, -60). The study demonstrates that the structure of α-amanitin in solution is similar to the structure f β-amanitin in the crystalline state.
    Additional Material: 5 Ill.
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