ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Column void volume
;
Cyclodextrin stationary phase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A new graphical method is proposed for the determination of the dead/void volume of liquid chromatographic columns with β-cyclodextrin stationary phase. Two different approaches are presented which lead to very similar dead volume values for the cyclodextrin columns. The validity of the proposed method is discussed on the basis of column porosity values, as well as the resulting linear relationship between the logarithm of the capacity factor and the number of carbon atoms in the n-alkanol homologs. The method was applied to study the influence of various experimental parameters on the dead volume of cyclodextrin columns.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02274535
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