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  • 1
    Electronic Resource
    Electronic Resource
    Molecular and functional characterization of a mutant allele of the mitogen-activated protein-kinase geneSLT2(MPK1) rescued from yeast autolytic mutants (1996)
    Springer
    Current genetics 29 (1996), S. 516-522 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; SLT2 ; MAP-kinase ; Caffeine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have further characterized the functionality of theSaccharomyces cerevisiae geneSLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-remediable phenotype oflyt2 mutants. Bothslt2A andlyt2 mutants displayed a caffeine-sensitive phenotype consisting of cell lysis that was not dependent on temperature. Caffeine concentrations affecting the growth of these mutant strains were dependent on the genetic background, theSSD1 allele being very significant in this regard. TheSLT2 allele of severallyt2 strains was both rescued and amplified by PCR. The recovered allele was shown to be non-functional as it could not complement the lytic phenotype of both deletion (slt2Δ) andlyt2 strains. After nucleotide sequencing of the recovered allele, we found that the defect oflyt2 mutants consists in a substitution of an aspartic acid for a glycine at position 35 of the amino-acid sequence of Slt2p. Gly35 is the third glycine of a glycine cluster (Gly-X-Gly-X-X-Gly), a conserved region in protein kinases and other nucleotide-binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02426955
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular and functional characterization of a mutant allele of the mitogen-activated protein-kinase gene SLT2(MPK1) rescued from yeast autolytic mutants (1996)
    Springer
    Current genetics 29 (1996), S. 516-522 
    Details
    ISSN: 1432-0983
    Keywords: Keywords Yeast ; SLT2 ; MAP-kinase ; Caffeine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-remediable phenotype of lyt2 mutants. Both slt2Δ and lyt2 mutants displayed a caffeine-sensitive phenotype consisting of cell lysis that was not dependent on temperature. Caffeine concentrations affecting the growth of these mutant strains were dependent on the genetic background, the SSD1 allele being very significant in this regard. The SLT2 allele of several lyt2 strains was both rescued and amplified by PCR. The recovered allele was shown to be non-functional as it could not complement the lytic phenotype of both deletion (slt2Δ) and lyt2 strains. After nucleotide sequencing of the recovered allele, we found that the defect of lyt2 mutants consists in a substitution of an aspartic acid for a glycine at position 35 of the amino-acid sequence of Slt2p. Gly35 is the third glycine of a glycine cluster (Gly-X-Gly-X-X-Gly), a conserved region in protein kinases and other nucleotide-binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050080
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  • 3
    Electronic Resource
    Electronic Resource
    Solute-micelle association constants and correlation of octanol-water coefficients with hydrophobicity for polycyclic aromatic hydrocarbons by micellar chromatography (1992)
    Springer
    Chromatographia 34 (1992), S. 627-635 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Micelle-solute association constants ; Polynuclear aromatic hydrocarbons ; Octanol/water partition coefficients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Sodium dodecyl sulphate, hexadecyltrimethylammonium bromide and polyoxyethylene(23) lauryl ether were investigated as mobile phases in the micellar liquid chromatography of polycyclic aromatic hydrocarbons of environmental concern. A wide range of surfactant concentrations were used and the retention parameters and distribution coefficients calculated. The trends in the retention data for the PAHs are discussed and several conclusions are presented concerning the partition coefficients when these surfactants are present in the mobile phase. Comparisons are made between the structural features of the compounds and their capacity factors, and the octanol/water partition coefficients are correlated with hydrophobicity for the PAHs studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269875
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  • 4
    Electronic Resource
    Electronic Resource
    Solute-micelle association constants of some polynuclear aromatic hydrocarbons by micellar liquid chromatography with alcohol additives to mobile phase (1994)
    Springer
    Chromatographia 38 (1994), S. 342-348 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Hybrid mobile phases containing alcohols ; Solute-micelle association constants ; Polynuclear aromatic hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The interaction between some polynuclear aromatic hydrocarbons of environmental concern, and sodium dodecylsulphate and hexadecyltrimethylammonium bromide in the presence of methanol, 2-propanol and n-butanol has been evaluated by high-performance liquid chromatography using micellar mobile phases. Solute-micelle association constants have been determined for the compounds investigated. The results show that the addition of the alcohol to the mobile phase decreases the association constant values, methanol 〈2-propanol 〈n-butanol, relative to those in the absence of any additive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269778
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