ISSN:
1432-203X
Keywords:
Key wordsBeta vulgaris
;
Agrobacterium rhizogenes
;
Transformation
;
Co-inoculation
;
Hairy roots
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A transformation protocol, based on co-inoculation with two strains of Agrobacterium, Agrobacterium tumefaciens LBA4404 and A. rhizogenes 15834 containing a binary vector with the GUS gene, was established for the induction of transgenic hairy roots from sugar beet (Beta vulgaris L.) explants. It resulted in marked improvement in the formation of hairy roots and the integration of the binary vector T-DNA into the host genome. Of 250 inoculated sugar beet hypocotyls, 84% yielded hairy roots 5–7 days after inoculation, of which 70% were co-transformed with the binary vector T-DNA. To determine stable expression of alien genes in hairy roots, the nematode resistance gene Hs1 pro-1 was used as a reporter gene. In addition, molecular marker analysis was applied to monitor stable incorporation of a translocation from the wild beet B. procumbens. The molecular analysis and the nematode (Heterodera schachtii) resistance test in vitro demonstrated that the genomic structure and the expression of the Hs1 pro-1 -mediated nematode resistance were well-maintained in all hairy root cultures even after repeated sub-culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050614
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