ISSN:
1432-072X
Keywords:
Clostridium cellobioparum
;
Clostridium thermocellum
;
Ammonium assimilation
;
Nitrogen fixation
;
Nitrogen control
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Inorganic nitrogen metabolism in two cellulose degrading clostridia, the mesophile Clostridium cellobioparum and the thermophile Clostridium thermocellum was investigated. Both strains show acetylene reduction (i.e. possibly nitrogenase activity), contain glutamine synthetase, glutamate dehydrogenase and glutamate-dependent transaminases. C. cellobioparum additionally contains a NADH-dependent glutamate synthase and a NH 4 + -repressible glycine dehydrogenase (NADPH). Remarkably, acetylene reduction in C. thermocellum is not repressed by ammonium, casting doubt whether this activity is due to nitrogenase. The results are compared with the data from other saccharolytic clostridia.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00446774
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