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  • 1
    Electronic Resource
    Electronic Resource
    Biophysical models of ciliary activity: Gaussian frequency distributions (1990)
    Springer
    European biophysics journal 18 (1990), S. 85-91 
    Details
    ISSN: 1432-1017
    Keywords: Ciliary activity ; Light scattering ; Gaussian beat frequency distribution ; Model fittings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract A model of a freely rotating exended scatterer is proposed to describe light scattering from beating cilia. Gaussian rotation frequency distributions, characterized by a mean angular frequency and a standard deviation, are introduced in order to simulate intensity autocorrelation functions and to fit the model to experimental data. Thus the ciliary beats are characterized by a mean beat frequency and a standard deviation of the beat frequency distribution. The standard deviation influences the damping of the intensity autocorrelation function of light scattered from cilia. The calculated intensity autocorrelation function shows a more prominent oscillating behaviour the smaller the standard deviation of the beat frequency. The validity of the model is supported by experimental data in two ways: 1) The model fits very well to experimental data in computer evaluations, 2) Neither the model nor information obtained from measurements are dependent on the measuring angle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00183267
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  • 2
    Electronic Resource
    Electronic Resource
    Submillisecond detection of single rhodamine molecules in water (1994)
    Springer
    Journal of fluorescence 4 (1994), S. 259-264 
    Details
    ISSN: 1573-4994
    Keywords: Fluorescence ; single molecules ; rhodamine 6G ; autocorrelation ; CW
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Using a modified confocal fluorescence microscope and a CW argon laser, we have measured fluorescence bursts from diffusing single Rh6G molecules that clearly exceed the background intensity. The exact average number of molecules in the observable volume elements was measured directly via the fluorescence intensity autocorrelation function. This allowed us to estimate the probability of finding several molecules simultaneously in the volume element. A tradeoff between the number of detected fluorescence photons and the signal-to-background ratio was observed. In a volume element of 0.24 fl, 4 photoelectrons on average were detected from a molecule of Rh6G with a fluorescence-to-background ratio of 1000, while the volume element of 60 fl yielded on average 100 photoelectrons with a background of 25 counts. In fast single-molecule detection the intersystem crossing into the triplet state plays an important role, affecting the maximum emission rate from the molecule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01878461
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