Publication Date:
1992-04-03
Description:
Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1 beta to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cerretti, D P -- Kozlosky, C J -- Mosley, B -- Nelson, N -- Van Ness, K -- Greenstreet, T A -- March, C J -- Kronheim, S R -- Druck, T -- Cannizzaro, L A -- New York, N.Y. -- Science. 1992 Apr 3;256(5053):97-100.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Immunex Corporation, Seattle, WA 98101.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1373520" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Base Sequence
;
Caspase 1
;
Cell Line
;
Chromosome Banding
;
*Chromosomes, Human, Pair 11
;
Cloning, Molecular
;
Enzyme Precursors/biosynthesis/*genetics/isolation & purification
;
Humans
;
Metalloendopeptidases/biosynthesis/*genetics/isolation & purification
;
Molecular Sequence Data
;
Neutrophils/enzymology
;
Oligodeoxyribonucleotides
;
Poly A/genetics/isolation & purification
;
Polymerase Chain Reaction/methods
;
RNA/genetics/isolation & purification
;
RNA, Messenger/genetics
;
Recombinant Proteins/biosynthesis/isolation & purification
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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