ISSN:
1617-4623
Keywords:
Spontancous mutations
;
Chromosomal deletions
;
27 min
;
Antibiotic resistance
;
l-serine effect
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Spontaneous mutants of Escherichia coli K12 displaying an increased level of the kanamycin resistance conferred by plasmid pGR71 were selected. Several mutants obtained in this way apparently carry large chromosomal deletions extending into galU and/or bglY (27 min). This positive selection of deletions allowed detection of a new locus located between galU and bglY. Deletions of this locus are responsible for increased resistance to kanamycin (Irk), decreased resistance to l-serine in minimal medium (Drs) and decreased resistance to chloramphenicol (Drc) when a cat gene is present in the bacteria.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331292
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