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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Environmental biology of fishes 32 (1991), S. 99-117 
    ISSN: 1573-5133
    Schlagwort(e): Molecular phylogeny ; Maximum parsimony ; Chondrichthyes ; Dipnoi ; Actinopterygii ; Tetrapoda ; Sarcopterygii
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Synopsis Approximately 98% of the sequence of the 18S ribosomal RNA (rRNA) of the coelacanth Latimeria chalumnae was determined by a combination of direct RNA sequencing and sequencing of rRNA genes amplified by the polymerase chain reaction. This sequence was compared with 18S rRNA sequences of similar length from seven other vertebrate species, representing the taxa Petromyzontiformes, Holocephali, Elasmobranchii, Actinopterygii, Dipnoi, Amphibia, and Amniota, in order to determine the most likely sister group of the coelacanth. Maximum parsimony analysis of these sequences resulted in a single most parsimonious tree containing a number of anomalous relationships among these groups. A bootstrap analysis showed that none of the relationships in this tree was significantly supported at the 95% level, however. Addition of data from 15 other vertebrates (providing multiple representatives of most of the higher taxa) resulted in similar ambiguous groupings, as did a number of methods of editing the sites compared (designed to eliminate rapidly evolving positions). These results may be due to a relatively rapid radiation of the major lineages of osteichthyans, the resolution of which will require molecular information from a larger portion of the coelacanth genome.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 49 (1992), S. 208-215 
    ISSN: 0730-2312
    Schlagwort(e): P2 promoter ; CAT ; glial cell ; plasmids ; SV40 ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Here we analyzed the effect of the suppressor proto-oncogene p53 on transcription from the P2 promoter of the murien c-myc gene. c-myc promoter constructs were coupled to the chloramphenical acetyl-transferase (CAT) gene and were transiently transfected into a human glial cell line along with plasmids overexpressing wild-type or mutant p53. It was found that significant repression of c-myc transcription took place following cotransfection with wild-type but not mutant p53. However wild-type p53 did not suppress transcription from the SV40 early promoter or from the MHC promoter. Promoter-CAT constructs containing only the ME1a2 or E2F elements, from the P2 promoter, were repressed by p53, indicating that p53 may exert its effect at these two sites within the P2 promoter. Finally, when the SV40 T antigen and wild-type p53 were expressed together in glial cells the repressive effect of p53 was abolished.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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