ISSN:
1615-6102
Keywords:
Chlorophyll-protein-complex
;
Chloroplast differentiation
;
Euglena gracilis
;
Glucose
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary In order to investigate the chloroplast reduction inEuglena gracilis, strain z during heterotrophic nutrition, the changes induced by glucose on the cellular amounts of chlorophyll, protein and carbohydrates are analysed as well as the variations of the size of chloroplasts and of the molecular composition of the thylakoids. At the 6th hour of the cell-cycle autotrophically cultured and with a light-dark-change of 14∶10 hours synchronizedEuglena gracilis was transferred to permanent light together with application of glucose (1%) (= photoheterotrophic conditions) or to permanent light only (= autotrophic conditions). After 5, 96, 120 or 144 hours intact chloroplasts were isolated, osmotically shocked and their thylakoids degraded stepwise by addition of sodium-deoxycholate. The proteins of the resulting three membrane-fractions were separated electrophoretically. The amount of the chlorophyll-protein-complexes was determined. After 140 hours photoheterotrophic nutrition the cellular amounts of chlorophyll and protein are diminished, in comparison with autotrophic cultivation, especially the content of the chlorophyll-protein-complex CP II decreases, which is related to the light-harvesting-complex, whereas the chlorophyll-protein-complex CP I—the monomer of the reaction centers of photosystem I—is unaffected.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01275562
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