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  • Chitin synthetase  (4)
  • FIA  (4)
  • Springer  (8)
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  • 1
    Digitale Medien
    Digitale Medien
    Activation of chitin synthetase from Phycomyces blakesleeanus by calcium and calmodulin (1987)
    Springer
    Archives of microbiology 148 (1987), S. 280-285 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Chitin synthetase ; Phycomyces blakesleeanus ; Calcium-calmodulin ; Proteases
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Levels of basal chitin synthetase in cell-free extracts from Phycomyces blakesleeanus were reduced by breakage of cells in the presence of EDTA or EGTA. Addition of Ca2+ to these extracts activated chitin synthetase. Maximal activation was obtained after 2 h at a Ca2+ concentration of 2–5 mM. Activation by calcium was not reduced by any protease inhibitor tested but benzamidine, whereas the weak proteolytic activity of the extracts was inhibited by antipain. Larger levels of chitin synthetase activation were obtained by the simultaneous addition of calcium and calmodulin in most, but not all extracts. This further activation by calmodulin was prevented by TFP. ATP or cAMP did not stimulate activation by calcium or calcium-calmodulin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00456705
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Separation of chitosomes and secretory vesicles from the “slime” variant of Neurospora crassa (1987)
    Springer
    Archives of microbiology 149 (1987), S. 156-162 
    Details
    ISSN: 1432-072X
    Schlagwort(e): “Slime” variant ; Neurospora ; Chitosomes ; Chitin synthetase ; Secretory vesicles ; Invertase ; Phosphatase ; Membranes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cells from the “slime” variant of Neurospora crassa were broken in isotonic conditions by use of triethanolamine buffer plus EDTA. After removal of large membranous structures by low-speed centrifugation, chitosomes and secretory vesicles were separated by means of gel filtration, precipitation of membranous contaminants with Concanavalin A, and centrifugation in sucrose or glycerol gradients. Polypeptidic composition of fractions enriched in secretory vesicles or chitosomes was found to be distinct. By these criteria we concluded that chitosomes and secretory vesicles represent different populations of microvesicles. Both microvesicular populations appeared free of endoplasmic reticulum and vacuolar contaminants as demonstrated by determination of appropriate enzymatic markers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00425082
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  • 3
    Digitale Medien
    Digitale Medien
    Stabilization of chitin synthetase and purification of chitosomes from several mycelial Mucorales (1987)
    Springer
    Antonie van Leeuwenhoek 53 (1987), S. 171-181 
    Details
    ISSN: 1572-9699
    Schlagwort(e): Chitin synthetase ; chitosomes ; Mucorales
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Stability of chitin synthetase in cell-free extracts from mycelial fungi was markedly improved by the presence of sucrose in the homogenization media. Breakage of mycelium in sucrose-containing buffer yielded enzyme preparations from which chitosomal chitin synthetase could be purified by a procedure involving ammonium sulfate precipitation, gel filtration and centrifugation in sucrose density gradients. Purified chitosomes catalyzed the synthesis of chitin microfibrils in vitro upon incubation with substrate and activators. Chitosomal chitin synthetase from the filamentous form of M. rouxii was similar to the enzyme from yeast cells, except for the poorer stability and diminished sensitivity to GlcNAc activation of the former.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00393845
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  • 4
    Digitale Medien
    Digitale Medien
    Alterations in the vesicular pattern and wall growth ofPhycomyces induced by the calcium ionophore A 23187 (1989)
    Springer
    Protoplasma 148 (1989), S. 15-25 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Vesicles ; Phycomyces ; Calcium ionophore ; Chitin synthetase ; Invertase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Hyphal elongation, chitin synthesis in vivo, and invertase secretion inPhycomyces blakesleeanus were all inhibited almost instantly by the addition of 5–10 μM calcium ionophore A 23187. Protein biosynthesis was inhibited in these conditions by 30–50%. The ionophore did not affect cell respiration for at least 40 min. Effect on chitin biosynthesis was not due to alterations of the chitin synthetase levels or its activity; nor to impairement in GlcNAc metabolism. In drug-treated cells the number of apical vesicles was severely reduced even at very short periods of incubation, and these low numbers remained constant for at least 60 min of incubation with the ionophore. We suggest that the ionophore collapses the cellular calcium gradient and/or interferes with the normal electrical transhyphal current. As a consequence, formation and migration of apical vesicles are inhibited. These results are further evidence of the role of vesicles in fungal tip growth and exhibit the fact that active chitin synthetase is short-lived in vivo demanding its continuous supply by chitosomes to the cell surface.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01403987
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  • 5
    Digitale Medien
    Digitale Medien
    Semiautomatic determination of furazolidone and furaltadone by continuous and stopped flow FIA methods (1993)
    Springer
    Microchimica acta 112 (1993), S. 31-39 
    Details
    ISSN: 1436-5073
    Schlagwort(e): furazolidone ; furaltadone ; hydrolysis ; FIA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract New methods are proposed for the semiautomatic determination of furazolidone and furaltadone by photometric flow injection or stopped flow FIA analysis, on the basis of their alkaline hydrolysis reactions. Linear calibration graphs between 1 and 30 μg/ml are obtained for both compounds, the detection limits for furazolidone and furaltadone being 0.20 and 0.26 μg/ml, respectively, by flow injection and 0.27 and 0.21 μg/ml by the stopped flow approach.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01243318
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  • 6
    Digitale Medien
    Digitale Medien
    FIA-fluorimetric determination of adrenaline in pharmaceutical formulations by oxidation with molecular oxygen (1998)
    Springer
    Microchimica acta 128 (1998), S. 187-190 
    Details
    ISSN: 1436-5073
    Schlagwort(e): fluorimetry ; FIA ; adrenaline ; pharmaceutical formulations
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The fluorimetric determination of adrenaline is carried out in a continuous-flow assembly and by means of the molecular dissolved oxygen. The sample solution merges with an NaOH stream, then the resulting mixture is heated at 73 °C and led to the flow-cell of the fluorimeter. The flow-assembly is very simple and the procedure is quick (107 samples h−1) reproducible (R.S.D. 0.6%), selective and suitable to be applied to determination of adrenaline in formulations. Calibrations graph are linear over the ranges 0.05–15 and 20–40 mgl−1.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01243048
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  • 7
    Digitale Medien
    Digitale Medien
    Flow-injection spectrophotometric determination of molybdenum and vanadium with carminic acid in the presence of cetyltrimethylammonium bromide (1995)
    Springer
    Microchimica acta 118 (1995), S. 203-211 
    Details
    ISSN: 1436-5073
    Schlagwort(e): carminic acid ; molybdenum ; vanadium ; tensoactives ; CTAB spectrophotometry ; FIA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The color-forming reactions of molybdenum(VI) and vanadium(IV) with carminic acid (CA) in the presence of cetyltrimethylammonium bromide (CTAB) were used in the flow-injection determination of these metals. The micellar medium enhances the spectrophotometric features of the Mo(VI)-CA and V(IV)-CA binary complexes, and the higher solubility of these complexes when the CTAB concentration is above critical micelle concentration permits the easy application of flow-injection systems. Under the optimum conditions, linear calibration graphs were obtained from 0.76 to 28.8 μg/ml for molybdenum and from 0.30 to 9.1 μg/ml for vanadium. The detection limits were 0.33 and 0.16 μg/ml for molybdenum and vanadium, respectively. The selectivity of the method was investigated and the procedure was successfully applied to the determination of molybdenum in steels and pharmaceuticals and of vanadium in petroleum products.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01244360
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  • 8
    Digitale Medien
    Digitale Medien
    FIA-Fluorimetric assembly for the determination of noradrenaline hydrochloride by a solid-phase reactor with immobilized hexacyanoferrate(III) (1997)
    Springer
    Microchimica acta 126 (1997), S. 69-72 
    Details
    ISSN: 1436-5073
    Schlagwort(e): noradrenaline hydrochloride ; FIA ; solid-phase reactor ; fluorimetry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract An FIA assembly provided with immobilized hexacyanoferrate(III) is proposed for the fluorimetric determination of noradrenaline hydrochloride. The oxidative reagent is immobilized by means of a strong anion-exchange resin. The FIA manifold is very simple and the calibration graph is linear over the range 0.5–75mgl−1 noradrenaline hydrochloride with an r.s.d of 0.88% (17 replicates) and a sample throughput of 84h−1. Foreign compounds such as NaCl, sucrose, lactose and sodium sulfate caused no significant errors. The procedure is applied to the determination of noradrenaline in a medicinal formulation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01242663
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