ISSN:
1432-041X
Keywords:
Balbiani ring
;
Cell-free translation
;
Chironomus messenger RNA
;
Secretory proteins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Cell-free translation of salivary gland RNA or of purified Balbiani ring RNA (75S) in a reticulocyte lysate system gives rise to high molecular weight translational products (HMTP). In addition to a common size (approx. 1×106 daltons) HMTP share imunogenic determinants with the giant secretory proteins of salivary glands. This suggests that HMTP correspond to in vivo secreted proteins and thus, corroborates the notion that 75S-RNA is the messenger for these proteins. The time course of HMTP synthesis and the lack of appearance of lower molecular weight components as translational products of 75S-RNA indicate that the synthesis of HMTP (and of secretory proteins) occurs in one piece by an uninterrupted process. HMTP are regarded the largest polypeptides so far synthesized in a cell-free system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00848691
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