ISSN:
1432-072X
Keywords:
Methanobacterium formicicum
;
Chemostat
;
Growth parameters
;
Hydrogenase
;
Formate dehydrogenase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Growth of Methanobacterium formicicum strain MF was studied in pH-stat batch cultures and formatelimited chemostat cultures. The maxium rate of methanogenesis from formate occurred at pH 7.6 and 43°C and the maximum specific growth rate constant (μm) was 0.08 h-1. The K s and maximum growth yield (Y s max ) were 3.5 mM formate and 1.4 (g dry wt) mol01 formate respectively, and the maintenance coefficient (m) was calculated as 6.8 mmol formate (g dry wt)-1 h-1. The efficiency of electron transport phosphorylation during formate metabolism assuming Y ATP of 10.5 g mol-1 was about 20%. The specific activities of hydrogenase and formate dehydrogenase increased slightly with dilution rate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00408027
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