ISSN:
0935-6304
Keywords:
Liquid chromatography, HPLC
;
HPLC columns
;
Packing procedure
;
Aqueous slurry
;
Zeta potential
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Successful packing of high quality HPLC columns is dependent upon a number of parameters. Especially when using slurry packing techniques, the absence of conglomerates of particles in the slurry and the wettability of the particles are important. The method described in this paper fulfills these conditions, imparting a ξ potential to the 5 and 10 μm silica particles by applying a pH gradient. This results in repulsive forces between the particles and hence leads to a well-controlled monodisperse situation. Water is used as a slurry and displacement liquid; it combines the advantages of excellent wetting properties and non-toxicity.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240050505
Permalink