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  • 1
    Electronic Resource
    Electronic Resource
    Calciotropic hormones raise the chemically detectable [Pi] in UMR 106-06 osteoblast-like cells (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 11 (1993), S. 25-34 
    Details
    ISSN: 0263-6484
    Keywords: Cellular inorganic phosphate ; hormone action ; intracellular metabolism ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Uptake of orthophosphate (Pi) by osteoblast-like cells is known to be stimulated by parathyroid hormone (PTH), but effects on intracellular [Pi] have not been investigated. Here we show in rat osteoblast-like cells (UMR 106-06) that PTH (10-11 to 10-7 M) increases both 32Pi uptake and cellular [Pi] by up to 50 per cent. 1,25 Dihydroxyvitamin D3 (1,25D) (10-12 to 10-6 M) and salmon calcitonin (CT) (10-12 to 10-6 g ml-1) also increased cellular [Pi] (by up to 60 per cent), but the percentage increases in total cellular 32Pi uptake were smaller. The effects of 1,25D were transient (observable at 80 min and 6 h but not 24 h), and were also observed with 24,25 dihydroxy- and 25 hydroxyvitamin D3. Transient degradation of organic phosphorus pools to Pi might contribute to this increased [Pi]. These pools remain to be identified but were not shown to be phospholipids. Foetal bovine serum also affected cellular [Pi]. Care is therefore needed in distinguishing direct hormonal effects on cellular [Pi] from indirect effects arising from changes in the rate of cell growth.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290110104
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  • 2
    Electronic Resource
    Electronic Resource
    Regulation of the phosphate (Pi) concentration in UMR 106 osteoblast-like cells: Effect of Pi, Na+ and K+ (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 11 (1993), S. 13-23 
    Details
    ISSN: 0263-6484
    Keywords: Cellular inorganic phosphate ; cellular orthophosphate ; intracellular metabolism ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Osteoblast-like cells possess Na-dependent transporters which accumulate orthophosphate (Pi) from the extracellular medium. This may be important in bone formation. Here we describe parallel measurements of Pi uptake and cellular [Pi] in such cells from the rat (UMR 106-01 and UMR 106-06) and human (OB), and in non-osteoblastic human fibroblasts (Detroit 532 (DET)). In UMR 106-01, cellular [Pi] was weakly dependent on extracellular [Pi] and higher than expected from passive transport alone. [32Pi]-uptake was inhibited by Na deprivation, but paradoxically increased on K deprivation. With Na, 87 per cent of cellular 32P was found in organic phosphorus pools after only 5 min. Na deprivation also decreased cellular [Pi], in both UMR 106-01 and DET, but the decrease was smaller than that in [32Pi]-uptake. Ouabain decreased [32Pi]-uptake and cellular [Pi] in DET, but not in UMR 106-01. Regulation of cellular [Pi] is therefore at least partly dependent on Na/Pi co-transport, but this does not seem to be an exclusive property of osteoblasts.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290110103
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  • 3
    Electronic Resource
    Electronic Resource
    The application of capillary SFC, packed column SFC, and capillary SFC-MS in the analysis of controlled drugs (1991)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 14 (1991), S. 537-541 
    Details
    ISSN: 0935-6304
    Keywords: Capillary SFC ; Packed column SFC ; Capillary SFC-MS ; Controlled drugs ; Modified mobile phase generation ; Negative temperature programming ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Multi-component mixtures of controlled drugs, drug impurities, and adulterants have been analyzed by capillary SFC-FID, packed column SFC-UV, and capillary SFC-MS. Isocratic packed column SFC has been performed with binary and ternary mobile phases using a single syringe pump. The combination of capillary SFC and double focusing MS is described with reference to MS source pressures and the spectra obtained. The use of negative temperature programming in SFC is described.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240140805
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  • 4
    Electronic Resource
    Electronic Resource
    Velocity and Drop Size Measurements in Fuel Sprays in a direct injection diesel engine (1990)
    Weinheim : Wiley-Blackwell
    Particle and Particle Systems Characterization 7 (1990), S. 160-168 
    Details
    ISSN: 0934-0866
    Keywords: Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Process Engineering, Biotechnology, Nutrition Technology
    Notes: High-pressure injection fuel sprays have been investigated in a single-cylinder research Diesel engine using the laser and phase Doppler anemometry techniques to measure fuel droplet velocities and sizes and their spatial and temporal distributions within the spray. The measurements relate to the determination of the droplets' axial velocity component and diameter on the spray centre line between 75 and 100 nozzle diameters downstream from a single-hole injector nozzle. The experimental program covered the use of both Diesel and a non-combusting alcohol fuel. Measurements at radial locations in the spray and at different fuel loads were also taken for the alcohol fuel. Temporally resolved measurements of droplet velocity and size are presented together with time  -  averaged values in such a way as to isolate the contributions from the leading and trailing regions of each spray and identify two different break  -  up mechanisms occurring as suggested by a conceptual model of the spray behaviour.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ppsc.19900070129
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  • 5
    Electronic Resource
    Electronic Resource
    Theory of electrophoretic separations. Part II: Construction of a numerical simulation scheme and its applications (1986)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 32 (1986), S. 215-223 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The mathematical model outlined in Part I is recast in a form suitable for numerical computation. The spatial derivatives are replaced by finite-difference expressions, which leads to a set of ordinary differential equations coupled to a set of nonlinear algebraic relations. This system is solved using existing integration techniques. The resulting algorithm simulates the characteristic behavior of the classical modes of electrophoresis, which is shown by examples involving moving boundary electrophoresis and isoelectric focusing. In the first example two different integration schemes are used and their accuracy and stability investigated. The second example illustrates the versatility of the methodology.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690320207
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  • 6
    Electronic Resource
    Electronic Resource
    Evaluation of diffusivities in electrolyte solutions using Stefan-Maxwell equations (1986)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 32 (1986), S. 291-296 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The Stefan-Maxwell equations have been used to predict single-electrolyte diffusion and ternary tracer diffusion in concentrated electrolyte solutions. Variations in diffusivity due to concentration have been shown to be related to the relaxation effect and viscosity changes of solution. Specific equations are presented to evaluate the ion-ion and ion-solvent interaction coefficients appearing in the Stefan-Maxwell equations. These equations, when tested against experimental data, gave an accurate description of the process at up to 4.0 M concentrations and for trace species mobilities differing by a factor of ten.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690320216
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  • 7
    Electronic Resource
    Electronic Resource
    Multicomponent diffusion in concentrated electrolyte solutions: Effect of solvation (1987)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 33 (1987), S. 436-443 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method is presented to estimate the diffusivities of electrolytes in concentrated multicomponent systems. The method is based on the Stefan-Maxwell flux equations. Explicit expressions have been developed for the phenomenological transport coefficients. These expressions incorporate the effects of solvation, viscosity, and ionic interactions. It has been shown that multicomponent diffusion behavior can be related to corresponding single-electrolyte and tracer diffusion cases. Thus, solvation effects are estimated from single-electrolyte data, and ion-ion interaction effects are obtained from tracer diffusion studies. The method has been tested for the ternary system NaCl-KCl, at concentrations of up to 3.0 M, and for corresponding single-electrolyte and tracer diffusion cases at concentrations of up to 4.0 M. In general, the method predicts phenomenological coefficient values within 1.5% of the experimental values for univalent systems.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690330309
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of particle surface roughness on particle interactions in concentrated suspensions (1987)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 33 (1987), S. 862-866 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690330521
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  • 9
    Electronic Resource
    Electronic Resource
    A dynamic model for the interaction of caustic reagents with acidic oils (1990)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 36 (1990), S. 233-241 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper describes the mechanisms and a quantative analysis of the interaction between a multicomponent acid mixture with a spectrum of caustic solutions. A physico-chemical model of the acidic oil/caustic system has been proposed which demonstrates the effect on dynamic interfacial tension (IFT) of variations in caustic concentration as well as changes in the initial composition and ionization properties of the constituent acids of the oleic phase. The model relies on the Langmuirian theory of interfacial sorption kinetics in addition to the Nernstian theory of convective diffusion. Pertinent kinetic and mass transfer parameters for all contributing surface-active species were determined from a recently proposed single-component dynamic model (Chiwetelu et al., 1988a). The validity of this multicomponent dynamic model was confirmed by the close agreement between predicted IFT and experimental data for a binary carboxylic acid system in contact with a broad range of caustic concentrations.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690360209
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  • 10
    Electronic Resource
    Electronic Resource
    CO oxidation on Pt: Variable phasing of inputs during forced composition cycling (1990)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 36 (1990), S. 1796-1806 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A combined experimental and theoretical investigation of the effect of forced feed composition cycling for CO oxidation on platinum has been performed. A novel approach to forced composition cycling was examined, in which the phase angle between the two input streams was varied. Reaction rate enhancement is shown to occur, and by varying the phasing of the feed streams it is possible to achieve a global maximum in the time-average reaction rate. This phenomenon can be explained quantitatively by a model based on an adsorbate-induced phase change of the Pt surface combined with CO adsorption self-exclusion. This mathematical model can also quantitatively describe the complex steady-state behavior (uniqueness-multiplicity transitions) observed for this reaction. The predictions of the model have been validated further through a detailed experimental study of the effects of feed flow rate, temperature, size of catalyst charge, and cycling frequency on the instantaneous and time-average conversions during forced cycling of the feed composition.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690361203
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