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  • 1
    Electronic Resource
    Electronic Resource
    Pilot plant isolation of transaldolase from Candida utilis (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 12 (1970), S. 321-331 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Through the use of pilot plant equipment, transaldolase types I, II, and III (from Candida utilis) have been separated and purified. The procedure includes a time sensitive solvent fractionation below 0°C, ion exchange chromatography, and crystalization. The enzyme yield represents a 41% recovery of crystalline type III and partially purified types I and II.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260120211
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  • 2
    Electronic Resource
    Electronic Resource
    Nomenclature in viscometry (1956)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 22 (1956), S. 186-186 
    Details
    ISSN: 0022-3832
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1956.1202210023
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of free zone electrophoresis of fixed erythrocytes performed in microgravity (1985)
    Weinheim : Wiley-Blackwell
    Electrophoresis 6 (1985), S. 3-9 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A free fluid zone electrophoresis experiment was performed in the microgravity environment of Space Shuttle flight STS-3 (March 1983). The experiment was designed to confirm observations made on the Apollo-Soyuz mission of 1975 and to test the effect of high red blood cell (RBC) concentration on free fluid electrophoresis. Photographic documentation of cell zone progression in one-hour separations of mixtures of formaldehyde-fixed human and rabbit erythrocytes (RBC), which were subjected to a field of approximately 13 V cm-1 in low ionic strength buffer, was analyzed. One of two columns contained 2 × 108 RBC ml-1 (low concentration), and the other contained 1 × 109 RBC ml-1 (high concentration). The observed and calculated leading edge displacements of the RBC in the two columns were in agreement, indicating the absence of unexpected effects of the reduced gravity environment. Post-flight analyses of the contents of the columns was not possible, and additional microgravity experiments are needed to evaluate the role of particle-particle interactions in concentrated suspensions undergoing electrophoresis.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150060103
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  • 4
    Electronic Resource
    Electronic Resource
    Combined capillary column gas chromatography negative ion chemical ionization mass spectrometry of prostanoidsA preliminary report of this study was made at the British Mass Spectrometry Society 12th Meeting, Cambridge, 1981. (1983)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1983), S. 83-88 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Electron capturing pentafluorobenzyl ester derivatives of prostanoids provide intense negative ion chemical ionization mass spectra. Fragmentation is directed almost entirely away from the prostanoid molecule and this provides intense [M - C6F5CH2]- ions. These ions are ideal for specific and sensitive quantitative selected ion monitoring analysis. The limits of sensitivity employing this technique are in the range 1-8 pg on column compared with 100-500 pg using the corresponding methyl ester derivative in the electron impact mode. The capillary column characteristics of the pentafluorobenzyl esters are suitable for the development of multiprostaglandin gas chromatographic mass spectrometric assays.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200100207
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  • 5
    Electronic Resource
    Electronic Resource
    Peptide studies using a fast atom bombardment high field mass spectrometer and data system. 1 - Sample introduction, data acquisition and mass calibration (1983)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1983), S. 324-333 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Conditions were established for analyzing as little as 5 pmol of an underivatized peptide delivered in a glycerol sample matrix as a thin film onto a gold-plated copper sample stage and then bombarded with xenon fast atoms. Calibration of the fast atom bombardment high field mass spectrometer and data system was achieved using cesium iodide/glycerol as a reference. Calibration at several accelerating potentials permitted a mass range from 393 to 5941 u. Several factors were examined that contribute to the quality of the mass spectrum: components within the glycerol such as other peptides, alkali salts, acid and reducing agents; the nature of the fast atom gas; concentration of the peptide delivered to the sample stage; and the effect of the sample stage and sample matrix on sensitivity.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200100504
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  • 6
    Electronic Resource
    Electronic Resource
    Peptide studies using a fast atom bombardment high field mass spectrometer and data system: 3 - negative ionization: Mass calibration, data acquisition and structural characterization (1983)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1983), S. 387-393 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Under negative ionization conditions, nominal mass calibration of the fast atom bombardment high field mass spectrometer and data system was accomplished using cesium iodide/glycerol as a reference. Mass calibration at -8 kV accelerating potential extends from m/z 387 to m/z 2170 using xenon fast atoms. Negative xenon FAB mass spectra for human angiotensin I and human gastrin I complement their positive fast atom bombardment spectra. Negative xenon fast atom bombardment spectra of underivatized peptides exhibit molecular proton-abstracted ion envelopes and structurally significant fragment ions. Peptide mixture analysis under negative xenon fast atom bombardment reveals peptide molecular ion envelopes of higher relative intensities than under positive xenon fast atom bombardment.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200100609
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  • 7
    Electronic Resource
    Electronic Resource
    Peptide studies using a fast atom bombardment high field mass spectrometer and data system. 2 - characteristics of positive ionization spectra of peptides, m/z 858 to m/z 5729 (1983)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1983), S. 408-419 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Sixteen peptides ranging in molecular weights from 858 to 5729 were examined under positive ionization fast atom bombardment conditions employing a high field magnet mass spectrometer and data system. The contributions of the polyisotopic elements to the molecular protonated ion envelope become important in the interpretation of the data at higher mass. For masses less than 2000 u, the most abundant ion within this envelope is the monoisotopic molecular protonated ion. Above 2000 u, the most abundant ion in the envelope is a polyisotopic molecular protonated ion. Characterization of the peptide requires identification of both the molecular protonated ion envelope and significant fragment ions. Partial spectra displaying both the molecular ion and significant fragment ions are presented for mastoparan (mol. wt = 1478), somatostatin (mol. wt = 1637), bovine parathyroid hormone (1-34) (mol. wt = 4106), and bovine insulin (mol. wt = 5729). A partial spectrum for bovine ribonuclease A (mol. wt = 13673) displayed significant fragment ions that identified the protein. The types of fragment ions included those that indicated the amino acid sequence and the location of disulfide bonds. The abundance of these ions appears to be influenced by the characteristics of the noble gas fast atom beam and the sample matrix.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200100705
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  • 8
    Electronic Resource
    Electronic Resource
    Size, charge and structural heterogeneity of Brucella abortus lipopolysaccharides demonstrated by two-dimensional gel electrophoresis (1986)
    Weinheim : Wiley-Blackwell
    Electrophoresis 7 (1986), S. 283-288 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Phenol extracted, alkali-treated lipopolysaccharide (aLPS) from vaccine strain (S19) Brucella abortus was demonstrated by two-dimensional gel electrophoresis to consist of at least ten silver staining, polydisperse analogues having different pIs. When tested on nitrocellulose immunoblots, all ten were antigenically reactive with bovine anti-B. abortus polyclonal sera, but only six reacted with anti-B. abortus O-antigen murine monoclonal antibody. Analogues focusing at different pIs were concluded to arise from differences in either core or O-antigen side chain structure or because of covalently bound protein. While not qualitatively different, aLPS from pathogenic B. abortus strain 2308 had lesser amounts of analogues 1, 2, 5, 6, and 8 than did aLPS from strain 19 (vaccine). The 2-D gel electrophoresis method was demonstrated to be of value in the analysis of aLPS from B. abortus and may be useful in the study of lipopolysaccharides from other sources.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150070608
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  • 9
    Electronic Resource
    Electronic Resource
    A new method for the detection of proteolytic activity in Pseudomonas lundensis after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 43-45 
    Details
    ISSN: 0173-0835
    Keywords: Proteolytic activity ; Protease ; Sodium dodecyl sulfate-polyacrylamide gel electrophoresis ; Pseudomonas lundensis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A new method for the visualization of proteolytic activity in cell culture supernatant from Pseudomonas lundensis after sodium dodecyl sulfate (SDS) - gel electrophoresis is described. Following conventional electrophoresis, the gel is washed in a methanol-containing buffer to facilitate partial removal of SDS. After incubation with 0.5% casein the gel is stained for protein with Coomassie Brilliant Blue R-250. Bands with proteolytic activity appear as clear areas in the gel against a blue-stained background. Molecular weight standards electrophoresed in the same gel stain more intensely than the background and allow determination of the molecular weights of the proteolytic components. The sensitivity of post-electrophoretic reactivation in SDS-gels was determined using trypsin as standard. A slight modification of the technique allowed detection of proteolytic activity in nondenaturing and in isoelectric focusing gels.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150160110
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  • 10
    Electronic Resource
    Electronic Resource
    Inhibition of monocyte luminol-dependent chemiluminescence by tetrahydrobiopterin, and the free radical oxidation of tetrahydrobiopterin, dihydrobiopterin and dihydroneopterin (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 6 (1988), S. 191-195 
    Details
    ISSN: 0263-6484
    Keywords: Macrophages ; monocytes ; radical oxidation ; pteridines ; tetrahydrobiopterin ; dihydrobiopterin ; dihydroneopterin ; chemiluminescence ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Luminol-dependent chemiluminescence of normal human monocytes activated by zymosan is demonstrated to be inhibited by tetrahydrobiopterin in a concentration-dependent manner. The reduced pterins tetrahydrobiopterin, dihydrobiopterin, and dihydroneopterin are all shown to be readily oxidized by the hydroxyl radical.The susceptibility of reduced pterins to free radical attack may explain the inhibition of chemiluminescence observed and an additional role of reduced pterins as free radical scavengers in tissues is considered.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290060307
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