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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 22 (1980), S. 919-928 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Trypsin was immobilized on sand using five different methods. Attempts were made to attach amino-functional groups onto sand using 3-aminopropyltriethoxysilane, hexamethylenetetramine, hexamethylenediamine, and melamine. Glutaraldehyde was used as a bifunctional agent in all the methods. Methods for the estimation of the proteolytic 1activity and protein content of immobilized trypsin were standardized. The maximum retained activity was observed for trypsin immobilized on sand via 3-aminopropytriethoxysilane and glutaraldehyde. Immobilized trypsin showed a shift in the pH optimum toward the acidic side over that of soluble trypsin in all five cases. The optimum temperature for both native and immobilized trypsin prepared by the silane-glutaraldehyde method was found to be 45°C. However, the pH and thermal stabilities of immobilized trypsin were observed to be better than that of the native enzyme.
    Additional Material: 5 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 22 (1980), S. 2449-2454 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 30 (1902), S. 406-408 
    ISSN: 0863-1778
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 44 (1905), S. 237-266 
    ISSN: 0863-1778
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 12 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 178 (1929), S. 329-336 
    ISSN: 0863-1786
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 27 (1982), S. 795-810 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The effect of the ionic strength of the solvent on the size-exclusion chromatography of narrow molecular weight dextrans and monodisperse sodium polystyrene sulfonates has been investigated. In both NaOH and NaCl solutions the elution volume of the sodium polystyrene sulfonates increased as the ionic strength was increased, while the elution volume of the dextrans decreased slightly as the ionic strength was increased. The elution data for various ionic strength solvents could not be described by the traditional universal calibration procedure of plotting the product of the intrinsic viscosity and the molecular weight vs. the elution volume. By using a modified calibration procedure which includes excluded volume effects, the elution data can be described for NaOH solution of moderate and high ionic strength. This modified calibration technique was unable to describe the elution data for very low ionic strength NaOH solutions and for the NaCl solutions. Possible explanations of the inability of straightforward size exclusion chromatographic calibration techniques to quantitatively described all the observed elution behavior are discussed.
    Additional Material: 12 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 25 (1980), S. 263-275 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A novel approach to the gel permeation chromatography (GPC) of nonderivatized cellulose is reported using Sepharose CL-6B as the column packing material, 0.5N NaOH as the eluent, and cadoxen as the cellulose solvent. The traditional approach to GPC of cellulose has been to convert the cellulose to its nitrate thereby making it soluble in the solvent tetrahydrofuran. The circumvention of the need to derivatize the cellulose in the new system results in considerable saving of time. The new system gives good fractionation for cellulose. It also provides excellent separation of polystyrene sulfonate and dextran standards thereby making the system amenable to calibration. The effect of the particle size distribution of the column packing material on the efficiency of separation is discussed. Potential applications for this new method include studies on both acidic and enzymatic hydrolysis as well as fine structure of cellulose, starch, and other polymers capable of forming stable alkaline solutions.
    Additional Material: 9 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 25 (1980), S. 1601-1618 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The effects of a number of chemically nonreacting organic on shrinkage and stress-strain properties of preoriented semicrystalline poly(ethylene terephthalate) filaments have been studied. The changes in shrinkage and mechanical properties are found to be highly dependent on time and temperature, as well as on tension applied on the yarn during the treatment. The changes brought about by the solvents at lower temperatures (i.e., below 100°C) are either comparable with or more than those produced by thermal treatments in air or silicone oil above 200°C. The possibility of using slovent treatment for setting polyester for dimensional stability at low temperature as an alternative to the normal dry-heat-setting method has been discussed.
    Additional Material: 14 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 26 (1981), S. 223-230 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Poly(ethylene terephthalate) filament yarn has been annealed in nitrogen atmosphere, in silicone oil, or in nonreacting organic solvents. Morphological changes resulting from the annealing processes have been followed by Raman spectroscopy. Crystallinity, as evidenced by the width of the 1730 cm-1 carbonyl stretching vibration, is shown to affect the macroscopic properties of these filaments. Furthermore, these spectroscopic results, together with shrinkage measured, suggest that the shrinkage and the crystallization processes in the polymer fiber proceed at different rates in different fluid media.
    Additional Material: 2 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 27 (1982), S. 2281-2293 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A system for the aqueous size exclusion chromatography (SEC) of starch hydrolysates has been developed. Sepharose CL is the chromatographic support material, and aqueous sodium hydroxide solution is the eluent. Various factors affecting the resolution of the proposed system are discussed. Support materials having small particle size and narrow particle size distribution are necessary for maximizing separation efficiency. The ionic strength of the eluent, however, has a negligible effect on separation efficiency. The fractionation range of the system has been broadened by connecting in series columns containing two different Sepharose-CL gel types according to the bimodal pore size distribution concept. Well-characterized sodium polystyrene sulfonate and dextran standards are used to calibrate this SEC system. The Coll-Prusinowski calibration procedure leads to a linear calibration curve over a wide molecular weight domain.
    Additional Material: 11 Ill.
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