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  • Chemistry  (3)
  • 1
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Recombinant murine amelogenins M179 and M166 were expressed in Escherichia coli and purified. The aggregation properties of these amelogenins have been investigated in aqueous solutions as well as acetonitrile-containing solutions using dynamic light scattering. Dynamic light scattering provides direct measurement of the translational diffusion coefficient and hydrodynamic radius, and of an estimate of the molecular weight. Polydispersity and statistical parameters of how to interpret the analysis are also provided. Amelogenin aggregation was examined in solutions of a range of pH, ionic strengths, and protein concentrations. It was shown that at pH 7.8-8 and ionic strength of 0.02-0.05M the M179 molecules form monodispersed aggregates with hydrodynamic radii ranging from 15 to 19 nm. Analysis of hydrodynamic radii and size distribution of M179 aggregates in acetonitrile-containing solvents compared to that in aqueous solutions indicated a primary role for hydrophobic interactions in the association process of amelogenin molecules to form aggregates. Comparison between the aggregates formed by M179 and M166, which lacks the hydrophilic carboxy-terminal 13 residue sequence of M179, suggested that the self-assembly of amelogenin molecules to form stable and monodisperse aggregates requires the presence of the hydrophilic carboxy-terminal sequence of M179. © 1994 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0006-3525
    Keywords: apatite ; amelogenin ; crystal growth ; tooth enamel ; molecular self-association ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: At the secretory stage of tooth enamel formation the majority of the organic matrix is composed of amelogenin proteins that are believed to provide the scaffolding for the initial carbonated hydroxyapatite crystals to grow. The primary objective of this study was to investigate the interaction between amelogenins and growing apatite crystals. Two in vitro strategies were used: first, we examined the influence of amelogenins as compared to two other macromolecules, on the kinetics of seeded growth of apatite crystals; second, using transmission electron micrographs of the crystal powders, based on a particle size distribution study, we evaluated the effect of the macromolecules on the aggregation of growing apatite crystals. Two recombinant amelogenins (rM179, rM166), the synthetic leucine-rich amelogenin polypeptide (LRAP), poly(L-proline), and phosvitin were used. It was shown that the rM179 amelogenin had some inhibitory effect on the kinetics of calcium hydroxyapatite seeded growth. The inhibitory effect, however, was not as destructive as that of other macromolecules tested. The degree of inhibition of the macromolecules was in the order of phosvitin 〈 LRAP 〈 poly(L-proline) 〈 rM179 〈 rM166. Analysis of particle size distribution of apatite crystal aggregates indicated that the full-length amelogenin protein (rM179) caused aggregation of the growing apatite crystals more effectively than other macromolecules. We propose that during the formation of hydroxyapatite crystal clusters, the growing apatite crystals adhere to each other through the molecular self-association of interacting amelogenin molecules. The biological implications of this adherence effect with respect to enamel biomineralization are discussed. © 1998 John Wiley & Sons, Inc. Biopoly 46: 225-238, 1998
    Additional Material: 6 Ill.
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  • 3
    Publication Date: 2021-06-26
    Description: Berger is one of the most significant products as fish paste, now produced of red meat and red meat waste with different qualities, does not have much nutritional value .Using fish as an alternative to red meat, diversify and enhance the quality of nutritional value and the ability to use for all ages in the community of the aims of this study were , in addition the combined use of a cultured marine species is effective and to raise the index of unsaturated fatty acids and amino acids in the final product .In this study the effects of different replacement of kilka meat instead of Silver carp in the preparation of the consolidated burger on the acceptance, as well as changes unsaturated fatty acids and poly factor that it considered during 5 months of the best treatment choice for industrial production to be introduced industry Therefore 4 treatments Burger compilation as follows: Treatment (control), combination of burger with %100 Silver carp meat , treatment 1, combination of burger with %100 Kilka meat, treatment 2, combination of burger with %75 + 25 Kilka and Silver carp meat , treatment 3, combination of burger with %50 + 50 Kilka and Silver carp meat , after adding the %5.6 flour and breadcrumbs, %6 onions, %5soy, %3.25tomato paste,% 0.15 lemon juice,%2.1 garlic and egg powder and spices and %1.2salt to treatments , after preparation and packaging of the kept at cold room(-18o c )was evaluated . According to treatment 3 test (50% Kilka meat and 50% Silver carp meat) with the other treatments had significant difference (p 〈0.05) and had higher levels of unsaturated fatty acid. Also the treatment2, control and 1 respectively are secondary priorities. According to the goals of this project that combine Kilka and Silver crap meat on preparation of fusion burger is to economies the final product , results within 5 months of product storage at - 18 ° C reflects the fact that product quality in the long term preservation of unsaturated fatty acids has been accepted .
    Keywords: Biology ; Chemistry ; Fisheries ; Fish Berger ; Polyen Index ; unsaturated fatty acids ; Caspian kilka ; silver carp ; Omega 3 ; Omega 6 ; Clupeonella cultriventris ; Hypophthalmichthys molitrix ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 43-50
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