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  • 1
    Electronic Resource
    Electronic Resource
    Mutations affecting activity and transport of haemolysin in Escherichia coli (1987)
    Springer
    Molecular genetics and genomics 206 (1987), S. 238-245 
    Details
    ISSN: 1617-4623
    Keywords: E. coli ; Haemolysin ; Mutants ; Functional domains of HlyA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Temperature-sensitive mutants that exhibit an altered haemolytic phenotype were isolated from Escherichia coli harbouring the plasmid pHly152. Complementation with recombinant plasmids carrying one of the four hly genes (C, A, B or D) allowed localization of the hly ts mutations. A ts mutation in hlyC leads to a pro→leu exchange in amino acid position 53 of HlyC. Two ts mutations in HlyA were found in positions 312 (ser→pro) and 315 (thr→ile). Both amino acid exchanges are located in the same hydrophobic domain of HlyA which extends from amino acids 299 to 327. Two different mutations were introduced by site-specific mutagenesis in this hlyA domain: one by an exchange of ala, val to asp, glu (positions 313, 314) altering the hydrophobicity of this region and another which removes most of this hydrophobic portion. Both mutants have entirely lost the haemolytic activity but the mutant haemolysins are still efficiently transported across both membranes when hlyB and hlyD are provided. Functional HlyC is not required for the transport of the mutant haemolysins. Two site-specific mutations at the N-terminal end of hlyA (one at amino acid position 2 leading to a thr→pro exchange and another deleting ile and thr at positions 4 and 5) also do not affect the transport of the altered haemolysins. The thr→pro exchange enhances the haemolytic activity of the corresponding mutant, whereas the ile, thr deletion exhibits little or no effect on the haemolytic activity. Removal of the last 37 amino acids from the C-terminal end of HlyA leads to a truncated haemolysin which retains its haemolytic activity but is not secreted by the HlyB and HlyD transport system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333579
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  • 2
    Electronic Resource
    Electronic Resource
    Extrachromosomale DNA in Bakterien (1973)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 85 (1973), S. 569-580 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In vielen Bakterienzellen treten zusätzlich zur chromosomalen DNA, welche die genetische Information der Zelle enthält, kleinere ringförmige DNA-Faktoren auf, die als Plasmide oder Episomen bezeichnet werden. Diese genetischen Elemente verleihen der Zelle zusätzliche biochemische Fähigkeiten. Beschrieben werden die Fertilitätsfaktoren (F und F′), die Antibiotika-Resistenz-Faktoren (R), die Colicinogenen Faktoren (Col), die Hämolytischen Faktoren (Hly) und andere extrachromosomale DNA-Systeme. Diese kleinen DNA-Moleküle lassen sich isolieren und eignen sich somit besonders, um Fragen der DNA-Replikation und der stabilen Etablierung von genetischem Material in der Bakterienzelle zu untersuchen.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19730851303
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  • 3
    Electronic Resource
    Electronic Resource
    Extrachromosomal DNA in Bacteria (1973)
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 12 (1973), S. 517-528 
    Details
    ISSN: 0570-0833
    Keywords: Extrachromosomal DNA ; Bacteria ; DNA ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In addition to chromosomal DNA carrying the genetic information of the cell, many bacterial cells contain smaller circular DNA factors known as plasmids or episomes. These genetic elements endow the cell with additional biochemical capabilities. The fertility factors (F and F′), the antibiotic resistance factors (R), the colicinogenic factors (Col), the hemolytic factors (Hly), and other extrachromosomal DNA systems are described. These small DNA molecules can be isolated, and are therefore particularly suitable for the investigation of DNA replication and the stable establishment of genetic material in the bacterial cell.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/anie.197305171
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