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  • 1
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 304-313 
    ISSN: 0006-3592
    Schlagwort(e): Zymomonas mobilis ; molasses ; fermentation ; ethanol ; osmolality ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A new osmotolerant mutant strain of Zymomonas mobilis was successfully used for ethanol production from beet molasses. Addition of magnesium sulfate to hydrolyzed molasses allowed repeated growth without the need of yeast extract addition. The kinetics and yields parameters of fermentation on media with different molasses concentrations were calculated. The anabolic parameters (specific growth rate, μ, and biomass yield, YX/S) were inhibited at elevated molasses concentrations while the catabolic parameters (specific ethanol productivity, qp, and ethanol yield, Yp/s) were not significantly affected. In addition to ethanol and substrate inhibition, osmotic pressure effects can explain the observed results.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 28 (1986), S. 1838-1844 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A carrier-supported mycelial growth of Penicillium chrysogenum was applied to penicillin fermentation system using celite as a support material. Hyphal growth through the pore matrices of the material showed strong anchorages and provided highly stable biofilm growth. With bioparticles developed in such a manner, both cell growth and penicillin production were observed to increase significantly compared to the conventional dispersed filamentous cultures. Maximum values of specific penicillin production rate were found to be constant regardless of the growth form. A three-phase fluidized-bed fermentor was designed and tested for penicillin production using the bioparticles. Two modes of operation, semicontinuous and repeated fed batch, of the fermentor were tried. It was noted that the overgrowth of free mycelia and the development of fluffy loose bioparticles caused poor mixing and made the fermentor operation quite difficult. Control of the bioparticle size and the extension of production phase were therefore considered important to maintain the reactor productivity at a desired level. From the results of repeated fed-batch operation it was found that the control of bioparticle size could be successfully achieved by phosphate-limiting culture condition. Penicillin production under this condition was also observed to be maintained at a high level (about 80% of the maximum) for at least 1 month.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 47 (1995), S. 696-702 
    ISSN: 0006-3592
    Schlagwort(e): Bacillis subtilis ; spore mutant ; fed-batch ; continuous culture ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make it possible to identify the peculiar characteristics of the spore mutants unobtainable in batch culture. Spore mutants can enhance subtilisin productivity and prolong subtilisin production time in fed-batch culture as well as enable us to use very low dilution rates (〈0.1 h-1) without losing productivity in continuous culture, thereby improving the conversion yield of the nitrogen source. At 0.05 h-1 the spollG mutant of Bacillus subtilis DB104 (Δnpr Δapr) (Emr) spollG (Bimr):: pMK101 (Cmr) showed a subtilisin yield about ten times higher than that from wild-type DB104 (Δnpr Δapr)::pMK101 (Cmr). © 1995 John Wiley & Sons, Inc.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 23 (1992), S. 329-338 
    ISSN: 0933-5137
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Maschinenbau
    Beschreibung / Inhaltsverzeichnis: Influence of grain size on mechanical properties and dry oscillating sliding wear of Al2O3-ceramicsSpecimens with average grain sizes varying between about 0.8 μm and 12 μm were produced by cold isostatic pressing of high purity Al2O3-powder followed by sintering between 1300°C and 1700°C. Hardness, Young's modulus, bending strength and fracture toughness were measured as a function of average grain sizes. Tribological tests were carried out on the different microstructures at normal laboratory air and room temperature by using a ring-on-block tribometer.Experimental results showed the dependence of mechanical properties on grain size, hardness and bending strength obeying a Hall-Petch type relation, approximately. Coefficient of friction was relatively independent of grain size under the test conditions used. However, wear intensity increased substantially if a critical grain size was surpassed. This was due to a change in mechanisms of material removal which was confirmed by scanning electron microscopical studies of the worn surfaces.
    Notizen: Aus hochreinem Al2O3-Pulver wurden durch kaltisostatisches Pressen und Variation der Sintertemperatur zwischen 1300°C und 1700°C Formkörper mit unterschiedlichen mittleren Korngrößen zwischen etwa 0,8 und 12 μm hergestellt. Untersucht wurde der Einfluß der Korngröße auf mechanische und tribologische Eigen-schaften.Die Ergebnisse zeigten, daß mechanische Eigenschaften wie Härte, E-Modul, Biegebruchfestigkeit und Bruchzähigkeit von der Korngröße bzw. den Sintertemperaturen abhingen, wobei im Fall der Härte und Bruchfestigkeit näiherungsweise eine Hall-Peteh-Beziehung vorlag. Während die Reibungszahl relativ unabhängig von der Korngröße war, trat in der Verschleißintensität aufgrund geänderter Mechanismen ein starker Anstieg mit zunchmender Korngröße auf. Rasterelektronenmikroskopische Untersuchungen zeigten, daß der Verschleiß durch plastische Verformung, Mikrobruch, Kornfragmentierung und Deckschichten beeinflußt wurde.
    Zusätzliches Material: 14 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 26 (1980), S. 168-170 
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 27 (1981), S. 527-528 
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 35 (1995), S. 1636-1642 
    ISSN: 0032-3888
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: Aspects of thermal, morphological, and rheological properties of biodegradable poly-D(-)(3-hydeoxybutyrate) (PHB) blended with poly(ethylene oxide) (PEO) have been studied. Thermal properties and morphology of the blends were examined by scanning electron microscopy and differential scanning calorimetry, respectively. A rotational theometer with parallel plate geometry was also adopted to investigate the rheological properties of these blends. In addition, dynamic ciscoelasticity was measured by a Rheovibron as functions of time and temperature. From these measurements, PHB and PEO were observed to be miscible in the melt state. In the case of the blend systen 80/20 PHB/PEO by weight, the vacant domains of the PHB were filled with PEO particles, and this morphological state enhanced the rheological properties. Furthermore, PHB and its blends were found to have high crystallinities, but to have unstable thermal behavior about Tm.
    Zusätzliches Material: 13 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    ISSN: 1040-0397
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The response properties of a new thiocyanate-selective solvent-polymeric membrane electrode are reported. The membrane contains (5,10,15,20-tetrakis(2,4,6-triphenylphenyl)porphyrinato)manganese(III) chloride (Mn[TPPP]CI) in a plasticized polyvinyl chloride matrix. The electrode deviates from the Hofmeister selectivity pattern by responding to thiocyanate preferentially over inorganic anions (perchlorate, chloride, iodide, and bromide) and several organic anions often present in biological samples (ascorbate, citrate, urate, and salicylate). The performance of this electrode as an ion-selective detector in a low-dispersion, flow-injection system is also evaluated. While selectivity in such a nonequilibrium flow arrangement is found to be somewhat different than that observed under equilibrium conditions, the electrode's response and selectivity is still adequate to allow for measurement of thiocyanate in biological samples. This application is demonstrated via the flow-injection analysis (FIA) detection of thiocyanate in 22 human saliva samples with good correlation to a classical colorimetric method (r = 0.981).
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Weinheim : Wiley-Blackwell
    Electroanalysis 3 (1991), S. 909-916 
    ISSN: 1040-0397
    Schlagwort(e): Indium ; potentiometry ; membrane ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The potentiometric anion responses of polymeric membranes doped with various indium (III)-porphyrin structures are examined. The appropriate membranes are prepared by incorporating 1.0 wt % of the In(III)-porphyrins in plasticized poly(vinyl chloride) films. When such membranes are incorporated within standard ion selective electrode bodies, the resulting electrodes display non-Hofmeister anion selectivity, with a particularly enhanced and super-Nernstian response to chloride. Among those porphyrins investigated, membranes doped with chloro(octaethylporphyrinato)indium(III) yield the largest and most reproducible potentiometric anion responses. These same membrane electrodes exhibit negligible response to protons (or hydroxide) in the range of pH 4.5-9.0. When utilized as a flow-injection detector in conjunction with a protein diluent/buffer stream, the In(III)-porphyrin based electrodes may be used to determine chloride levels in human serum samples.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 55 (1997), S. 864-879 
    ISSN: 0006-3592
    Schlagwort(e): Corynebacterium glutamicum mutants ; transconjugation ; intracellular flux analysis ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The physiology and central carbon metabolism of Corynebacterium glutamicum was investigated through the study of specific disruption mutants. Mutants deficient in phosphoenolpyruvate carboxylase (PPC) and/or pyruvate kinase (PK) activity were constructed by disrupting the corresponding gene(s) via transconjugation. Standard batch fermentations were carried out with these mutants and results were evaluated in the context of intracellular flux analysis. The following were determined. (a) There is a significant reduction in the glycolytic pathway flux in the pyruvate kinase deficient mutants during growth on glucose, also evidenced by secretion of dihydroxyacetone and glyceraldehyde. The resulting metabolic overflow is accommodated by the pentose phosphate pathway (PPP) acting as mechanism for dissimilating, in the form of CO2, large amounts of accumulated intermediates. (b) The high activity through the PPP causes an overproduction of reducing power in the form of NADPH. The overproduction of biosynthetic reducing power, as well as the shortage of NADPH produced via the tricarboxylic acid cycle (as evidenced by a reduced citrate synthase flux), are compensated by an increased activity of the transhydrogenase (THD) enzyme catalyzing the reaction NADPH + NAD+↔NADP+ + NADH. The presence of active THD was also confirmed directly by enzymatic assays. (c) Specific glucose uptake rates declined during the course of fermentation and this decline was more pronounced in the case of a double mutant strain deficient in both PPC and PK. Specific ATP consumption rates similarly declined during the course of the batch. However, they were approximately the same for all strains, indicating that energetic requirements for biosynthesis and maintenance are independent of the specific genetic background of a strain. The above results underline the importance of intracellular flux analysis, not only for producing a static set of intracellular flux estimates, but also for uncovering changes occurring in the course of a batch fermentation or as result of specific genetic modifications. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55:864-879, 1997.
    Zusätzliches Material: 16 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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