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  • Chemical Engineering  (20)
  • thema EDItEUR::N History and Archaeology::NH History  (14)
  • Saccharomyces cerevisiae
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  • 1
    Digitale Medien
    Digitale Medien
    Sequence and transcription of the β-glucosidase gene of Kluyveromyces fragilis cloned in Saccharomyces cerevisiae (1987)
    Springer
    Current genetics 12 (1987), S. 175-184 
    Details
    ISSN: 1432-0983
    Schlagwort(e): β-glucosidase ; Kluyveromyces fragilis ; DNA sequence ; Saccharomyces cerevisiae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The complete nucleotide sequence of the β-glucosidase gene of Kluyveromyces fragilis has been determined. This sequence contains an open reading frame of 2535 base pairs encoding a protein of 845 amino acids. Analysis of the transcription products revealed only one transcript of about 3 kb identical in both Kluyveromyces fragilis and in the expression host Saccharomyces cerevisiae. The protein molecular weight of 93,811 Kd deduced from the sequence is consistent with the 90,000 Kd determined by SDS polyacrylamide gel electrophoresis with the purified protein. Mapping of the starts of transcription shows that two starting points are used in the natural host Kluyveromyces fragilis. A comparison of the amino acid sequence with that of other β-glucosidases revealed three regions of homology. One of these regions contains an amino acid sequence very similar to a peptide isolated from the active site of β-glucosidase A3 from Aspergillus wentii and could be implicated in the catalytic mechanism of these glucolytic enzymes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00436876
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  • 2
    Digitale Medien
    Digitale Medien
    Construction of new yeast vectors and cloning of the nif (nitrogen fixation) gene cluster of Klebsiella pneumoniae in yeast (1981)
    Springer
    Current genetics 3 (1981), S. 173-180 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Saccharomyces cerevisiae ; Yeast vectors ; Cosmids ; nif genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Two vectors, termed pG63.11 (7.6 Kb) and pHCG3 (9.6 Kb), suitable for yeast transformation have been constructed. The pHCG3 vector has cosmid properties. Both vectors contain a single 3.3 Kb EcoRI-HindIII fragment of yeast origin which carries the yeast URA3 gene (1.1 Kb) and the origin of replication of the 2 µm plasmid (2.2 Kb). They confer ampicillin resistance and they contain 5 unique EcoRI,HpaI,HindIII,BamHI and SalI restriction sites. Cosmid pHCG3 was used to clone the nitrogen fixation (nif) gene cluster of Klebsiella pneumoniae carried by twoHindIII fragments of 17 and 26 Kb, respectively. The resulting cosmid, termed pGPC875 (53 Kb) which conferred a Nif+ phenotype to Escherichia coli, was introduced in yeast by transformation. No acetylene reduction activity was detectable in the transformants. However it was shown that the entire information for nitrogen fixation can be replicated and maintained intact in yeast for more than 50 generations of growth.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00429819
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  • 3
    Digitale Medien
    Digitale Medien
    PDR3, a new yeast regulatory gene, is homologous toPDR1 and controls the multidrug resistance phenomenon (1994)
    Springer
    Molecular genetics and genomics 244 (1994), S. 501-511 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Saccharomyces cerevisiae ; Transcription factor ; Zinc finger ; Multidrug resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract TheSaccharomyces cerevisiae PDR3 gene, located near the centromere of chromosome II, has been completely sequenced and characterised. Mutationspdr3-1 andpdr3-2, which confer resistance to several antibiotics can be complemented by a wild-type allele of the PDR3 gene. The sequence of the wild-typePDR3 gene revealed the presence of a long open reading frame capable of encoding a 976-amino acid protein. The protein contains a single Zn(II)2Cys6 binuclear-type zinc finger homologous to the DNA-binding motifs of other transcriptional activators from lower eukaryotes. Evidence that the PDR3 protein is a transcriptional activator was provided by demonstrating that DNA-bound LexA-PDR3 fusion proteins stimulate expression of a nearby promoter containing LexA binding sites. The use of LexA-PDR3 fusions revealed that the protein contains two activation domains, one localised near the N-terminal, cysteine-rich domain and the other localised at the C-terminus. The salient feature of the PDR3 protein is its similarity to the protein coded byPDR1, a gene responsible forpleiotropicdrugresistance. The two proteins show 36% amino acid identity over their entire length and their zinc finger DNA-binding domains are highly conserved. The fact that the absence of both PDR1 and PDR3 (simultaneous disruption of the two genes) enhances multidrug sensitivity strongly suggests that the two transcriptional factors have closely related functions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00583901
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  • 4
    Digitale Medien
    Digitale Medien
    The bI4 RNA mitochondrial maturase of Saccharomyces cerevisiae can stimulate intra-chromosomal recombination in Escherichia coli (1989)
    Springer
    Molecular genetics and genomics 216 (1989), S. 70-74 
    Details
    ISSN: 1617-4623
    Schlagwort(e): RNA splicing ; Maturase ; Recombinase ; Mitochondria ; Saccharomyces cerevisiae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary When the bI4 RNA maturase, encoded by the fourth intron of the mitochondrial cytochrome b gene of Saccharomyces cerevisiae, was expressed in Escherichia coli, formation of intra-chromosomal Lac+ recombinants was stimulated threefold. This “hyper-rec” phenotype was recA as well as recBCD dependent. The most active form of the bI4 maturase stimulated homologous recombination whereas splicing deficient mutants of bI4 maturase were either deficient in or unable to stimulate homologous recombination.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00332232
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  • 5
    Digitale Medien
    Digitale Medien
    Evaluation of paper properties of thermomechanical pulp modified by ozonation and grafting with thermoplastic polymersPresented at Second World Congress of Chemical Engineering, Montréal, Canada, Oct. 4-9, 1981 (1984)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 24 (1984), S. 950-955 
    Details
    ISSN: 0032-3888
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: Paper properties of the thermochemical pulp modified by ozonation and grafting with styrene, acrylamide or their mixtures have been studied. Paper sheets, prepared by hot pressing from ozonated and grafted pulps showed improvement in: wet breaking length; dry breaking length; burst and dimensional stability.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pen.760241203
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  • 6
    Digitale Medien
    Digitale Medien
    Glass fiber-filled thermoplastics. II. Cavity filling and fiber orientation in injection molding (1985)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 25 (1985), S. 1008-1016 
    Details
    ISSN: 0032-3888
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: Mold filling of a rectangular cavity of three different thick nesses fed from a reservoir is studied for unfilled and glass fiber-filled polypropylene and polystyrene. The shapes of flow fronts studied by short-shots are affected predominantly by the thickness of the cavity with other parameters playing a less important role. Pressure drop versus volumetric flow rate inside the thinnest cavity is studied experimentally and predictions are made from a computer simulation of mold filling. The orientation of fibers in the cavity is examined using a reflect-type microscope and the orientation is found to depend on cavity thickness, melt temperature, fiber content, and to a lesser extent, on volumetric flow rate. In the thinnest cavity, where the flow is quasi-unidirectional, the fibers remain in the plane of flow oriented either along the flow direction or perpendicular to it, except in the region near the flow front, where they follow a “fountain” flow behavior.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pen.760251604
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  • 7
    Digitale Medien
    Digitale Medien
    Glass fiber-filled thermoplastics. I. Wall and processing effects on rheological properties (1985)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 25 (1985), S. 1001-1007 
    Details
    ISSN: 0032-3888
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: A systematic study of wall effects on the shear viscosity of short glass fiber-filled polypropylene and polystyrene is presented. The dependence of these effects on capillary radius, shear rate, temperature, and polymer matrix is examined. The “true” viscosity curves of these materials (free from wall effects) can be obtained by an extrapolation procedure. Breakage of glass fibers in the high shear-rate processes of extrusion and injection molding lead to an appreciable reduction of the viscosity of these materials and is probably the more important effect to take into account in these processes.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pen.760251603
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  • 8
    Digitale Medien
    Digitale Medien
    Volumetric heating by electromagnetic energy absorption - a potential for the mass production of structural composites (1993)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 33 (1993), S. 240-247 
    Details
    ISSN: 0032-3888
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: The problem of excessive heat buildup in a heat diffusion supported thermosetting process may lead, especially in the case of thick composite profiles, to compromised quality or even thermal degradation of the final product. A computer simulation of a real manufacturing process demonstrates this intrinsic weakness of the conventional manufacturing process by showing nonuniformities in the degree of cure development and a temperature inversion at the end of the processing cycle. Those nonuniformities in thermal expansion and contraction may be avoided by heating the composite uniformly throughout the whole cross section. This can be achieved with the help of electromagnetic irradiation. The electric energy absorption and, hence, conversion to heat takes place uniformly in the whole bulk of the material, regardless of the position. In effect, one can expect that the uniformly fast cured composite features superior mechanical properties over that of a composite that is conventionally processed.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pen.760330407
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  • 9
    Digitale Medien
    Digitale Medien
    Rheological characterization of fast-reacting thermosets through spiral flow experiments (1992)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 32 (1992), S. 172-181 
    Details
    ISSN: 0032-3888
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: The standard spiral flow test is normally used to assess the “moldability” of thermosetting materials. It is proposed that such tests can be modified and analyzed to yield rheological information as from a viscometer. Specifically, a “parameter-identification” scheme is developed to determine the constants in a viscosity model by minimizing the difference between the outcome of spiral flow tests and the results of numerical simulations of these tests. The proposed scheme is particularly applicable to fast-reacting thermosets for which typical viscometers cannot be used. Successful application of the scheme is demonstrated for a particular encapsulating epoxy material, where constants are obtained for a modified WLF equation viscosity model. These constants are used to predict the outcome of spiral flow experiments not involved in the minimization procedure. A favorable comparison between experiment and simulation occurs at most operating conditions. The method requires no elaborate equipment or delicate instrumentation.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pen.760320303
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  • 10
    Digitale Medien
    Digitale Medien
    Dense-gas solvent-solute clusters at near-infinite dilution: EPR spectroscopic evidence (1993)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 39 (1993), S. 876-884 
    Details
    ISSN: 0001-1541
    Schlagwort(e): Chemistry ; Chemical Engineering
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The nitrogen hyperfine splitting constant of ditertbutyl nitroxide radicals was measured with electron paramagnetic resonance spectroscopy at near-infinite dilution in near-critical and supercritical ethane, as well as in liquid propane, liquid isobutane, and several nonhydrogen bonding liquid solvents. While the measurements in the liquids are described well by the theory of McRae, large deviations from the liquid behavior are observed in supercritical ethane. The deviations are used as a measure of the effective local density of the solvent around the solute. At the two temperatures investigated, Tr = 1.009 and Tr = 1.084, the local density enhancement, defined as the ratio of local to bulk densities, exhibits a maximum of about 3 occurring around 1/2 the critical density. The maximum is removed well from the critical density, where the maximum of the isothermal compressibility is observed. Local density enhancements are short-range effects and do not correlate well with the development of long-range critical phenomena. Local density enhancement data in ethane are compared with the prewetting transition that has been observed in nearcritical ultrapure argon.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/aic.690390516
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