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  • 1
    Electronic Resource
    Electronic Resource
    Glycosylation inhibits the interaction of invertase with the chaperone GroEL
    Amsterdam : Elsevier
    FEBS Letters 305 (1992), S. 203-205 
    Details
    ISSN: 0014-5793
    Keywords: Aggregation ; Chaperone ; Folding ; Glycosylation ; GroE ; Invertase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(92)80667-6
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  • 2
    Electronic Resource
    Electronic Resource
    Comparison of the expression of a plastidic chaperonin 60 in different plant tissues and under photosynthetic and non-photosynthetic conditions (1996)
    Springer
    Planta 200 (1996), S. 326-334 
    Details
    ISSN: 1432-2048
    Keywords: Chaperone ; Chloroplasts ; Chromoplasts ; Heat-shock protein ; Secale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A partial cDNA which codes for the β-subunit of a plastidic chaperonin 60 (cpn60-β) from rye (Secale cereale L.) leaves was identified and sequenced, except for 46 amino acids of the N-terminus of the mature protein and the transit sequence. This is the first cpn60-β sequence determined for a monocotyledonous plant. Specific antibodies against cpn60-β were affinity-purified from an antiserum raised against the total soluble protein fraction of ribosome-deficient plastids. The localization of cpn60-β in chloroplasts or non-green plastids was confirmed by immunodetection in Percoll gradient-purified organelles. The expression and occurrence of cpn60-β was analysed by immunoblotting with the specific antibodies and Northern hybridization. The cpn60-β protein was constitutively expressed in various green and non-green tissues. It was evenly distributed along the major part of a rye leaf, while highest transcript levels occurred in the youngest and oldest leaf sections. The expression of the cpn60-β protein was not enhanced by a heat-shock treatment at 42 °C. The cpn60-β transcript and protein were more strongly expressed in various non-green, for instance etiolated, 70S-ribosome-deficient 32 °C-grown, or herbicide-bleached tissues, than in green leaves of rye. A rapid increase in the cpn60-β transcript level was also observed when green leaves were transferred from light to darkness while the protein level was not affected. The dark-induced increase in the cpn60-β transcript was totally suppressed in the presence of 2% sucrose. Inhibitor treatments suggested that the change in cpn60-β transcript level was not related to changes of the ATP supply of the tissue. While the large subunit of the photosynthetic protein ribulose-1,5-bisphosphate carboxylase was largely degraded during ripening of tomato fruits, high levels of cpn60-β were detected in tomato chromoplasts and in the yellow flower petals of Narcissus. Low levels of cpn60-β were detected in root tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00200300
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitative analysis of ethylenediamine in plasma by capillary column gas chromatography (1986)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 9 (1986), S. 548-554 
    Details
    ISSN: 0935-6304
    Keywords: Gas liquid chromatography (GLC) ; Fused silica capillary columns ; Alkali flame ionization detection (AFID) ; Mass spectrometry (MS) ; Quantitation in human serum ; Ethylenediamine ; Aminophylline ; Small molecular weight amines and diamines ; m-Toluoyl derivatives ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A specific and sensitive method for the determination of ethylenediamine in human plasma is described. The aqueous sample is treated with m-toluoyl chloride, yielding the N,N′-bis(m-toluoyl) derivative of the diamine, which is extracted into dichloromethane and quantitated after “on column” methylation by capillary gas liquid chromatography with alkali flame ionization detection. The corresponding derivative of putrescine serves as internal standard. The assay is reproducible and calibration curves are linear over the concentration range 0.05 to 10 μg · ml-1. The lower detection limit is about 10 ng · ml-1. The structures of the compounds of interest eluting from the capillary column are examined by gas liquid chromatography/mass spectrometry. The assay has been applied to the analysis of ethylenediamine in plasma following the administration of aminophylline and ethylenediamine in a cross-over study to patients with bronchopulmonary diseases. The method also proves suitable for measuring other primary and secondary amines and diamines in aqueous solutions by gas liquid chromatography.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240091002
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  • 4
    Electronic Resource
    Electronic Resource
    Infrared and Raman studies on calcium, zinc and cadmium hydroxide halides Ca{O(H,D)}Cl, Cd{O(H,D)}Cl, Zn{O(H,D)}F and β-Zn{O(H,D)}ClContribution LXXIX in a series of papers on lattice vibration spectra. For Part LXXVIII, see K. Beckenkamp, H. D. Lutz, H. Jacobs and U. Metzner, J. Solid State Chem. in press. (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 24 (1993), S. 797-804 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: IR and Raman spectra of Zn(OH)F, β-Zn(OH)Cl and isostructural Ca(OH)Cl and Cd(OH)Cl and of deuterated specimens recorded at ambient and liquid nitrogen temperature are presented and assigned to OH(OD) stretching, librational and translational modes. The spectra obtained are discussed on the basis of the layered crystal structures of these hydroxide halides. The main results are (i) detection and proof of the presence of very weak hydrogen bonds in the case of M(OH)Cl (M = Ca, Cd) and for OH(2) of Zn(OH)F [in addition to ordinary hydrogen bonds in the case of β-Zn(OH)Cl and OH(1) of Zn(OH)F] with OH stretching wavenumbers of the corresponding hydroxide ions partially greater than that of free, gaseous OH- ions, (ii) a less symmetrical structure of Ca(OH)Cl and Cd(OH)Cl compared with that obtained from the heavy atoms alone and (iii) proof of the presence of two different kinds of hydroxide ions in nearly equal amounts in Zn(OH)F containing two lattice sites each occupied by F- and OH-. The high-energy shift of the OH modes mentioned above is attributed to the synergetic effect.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250241113
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