ISSN:
1432-2242
Keywords:
Key words Microsatellite
;
Sequence variation
;
Genetic diversity
;
Cereals
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In an attempt to study changes associated with microsatellites in rice, the DNAs of cultivated rice, including indica and japonica varieties, and wild rice genotypes were amplified by the polymerase chain reaction with primers flanking the (GATA) n and (AC) n repeats at a microsatellite-containing locus OS1E6 (Genebank accession number AFO16647) previously reported from a PstI rice (var. Malkolam) genomic library in pUC18. Eight alleles of varying sizes were obtained which were cloned and sequenced. Sequencing data indicated that the size variations of the different alleles were due to differences in the repeat number as well as to sequence variations in the region flanking the microsatellite motifs. In order to study the presence of this complex microsatellite-containing locus of rice in different cereals, their DNAs were amplified using primers flanking the OS1E6 locus. It was found that this locus was present in the various cereal genotypes analyzed, indicating its conservation across different cereal members.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220051609
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