ISSN:
1615-6102
Keywords:
Microtubule
;
Plasma membrane
;
Cellular morphogenesis
;
Allogromia
;
Foraminifera
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Video-enhanced microscopy was used to study the behavior of cytoplasmic microtubules in flattened reticulopodia of the marine protistAllogromia. Linear microtubule bundles were observed bending to various degrees and then straightening. When microtubules bent sufficiently to contact the plasma membrane, protuberances extended from the pseudopodial margins. These protuberances withdrew as the bent microtubules straightened. In extreme cases, microtubules formed c-shaped loops which moved laterally through the cytoplasm and contacted fenestrae formed within the flattened pseudopodia. A given fenestra first deformed at the site of microtubule contact and then closed as the loop continued its motion; reversal of the microtubule motion reopened the fenestra. By electron microscopy, microtubules are consistently seen within 20 nm of the plasma membrane and are often connected to the membrane by detergent-resistant crosslinks. Together, these observations indicate that microtubule movements can deform the plasma membrane and thus mediate certain aspects of cellular morphogenesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01539847
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