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  • Cells, Cultured  (54)
  • American Association for the Advancement of Science (AAAS)  (54)
  • 1980-1984  (54)
Collection
Keywords
Publisher
  • American Association for the Advancement of Science (AAAS)  (54)
Years
Year
  • 1
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    Bioactive conformation of luteinizing hormone-releasing hormone: evidence from a conformationally constrained analog (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-11-07
    Description: An analog of luteinizing hormone-releasing hormone containing a gamma-lactam as a conformational constraint has been prepared with the use of a novel cyclization of a methionine sulfonium salt. The analog is more active as a luteinizing hormone-releasing hormone agonist that the parent hormone, and provides evidence for a bioactive conformation containing a beta-turn.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Freidinger, R M -- Veber, D F -- Perlow, D S -- Brooks, J R -- Saperstein, R -- New York, N.Y. -- Science. 1980 Nov 7;210(4470):656-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7001627" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Biological Assay ; Cells, Cultured ; Female ; *Gonadotropin-Releasing Hormone/analogs & derivatives ; Hydrogen Bonding ; Lactams ; Protein Conformation ; Rats ; Structure-Activity Relationship
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Specific chromosome defect associated with human small-cell lung cancer; deletion 3p(14-23) (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-01-08
    Description: A specific, acquired chromosomal abnormality (deletion 3p) has been found in at least one chromosome 3 in 100 percent of the metaphases in 12 of 12 cell lines cultured from human small-cell lung cancer tissue and in 2-day tumor culture specimens from three patients. Analysis of the shortest region of overlap shows the deletion to be 3p(14-23). This specific change was not seen in five of five lung cancer cell lines other than small-cell lung cancer or in two lymphoblastoid lines cultured from cells of small-cell lung cancer patients whose tumors had the 3p deletion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Whang-Peng, J -- Kao-Shan, C S -- Lee, E C -- Bunn, P A -- Carney, D N -- Gazdar, A F -- Minna, J D -- New York, N.Y. -- Science. 1982 Jan 8;215(4529):181-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6274023" target="_blank"〉PubMed〈/a〉
    Keywords: Carcinoma, Small Cell/*genetics ; Cells, Cultured ; *Chromosome Deletion ; Chromosomes, Human, 1-3 ; Humans ; Karyotyping ; Lung Neoplasms/*genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    Immunocytochemically identified vasopressin neurons in culture show slow, calcium-dependent electrical responses (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-09-09
    Description: From morphological characterization and intracellular recordings, monolayer cultures derived from fetal mouse hypothalami were found to include functionally differentiated peptide neurons, a number of which appear to contain vasopressin. These cells exhibited particular patterns of slow, calcium-dependent membrane depolarizations, resembling in their periodicity and duration the phasic activity of vasopressin neurons recorded extracellularly in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Theodosis, D T -- Legendre, P -- Vincent, J D -- Cooke, I -- New York, N.Y. -- Science. 1983 Sep 9;221(4615):1052-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6348947" target="_blank"〉PubMed〈/a〉
    Keywords: Action Potentials ; Animals ; Calcium/*pharmacology ; Cells, Cultured ; *Electrophysiology ; Histocytochemistry ; Hypothalamus/analysis/*cytology ; Immunologic Techniques ; Mice ; Neurons/analysis ; Vasopressins/*analysis
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    Transporting renal epithelium: culture in hormonally defined serum-free medium (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-11-21
    Description: A hormonally defined medium was used to isolate a homogeneous epithelioid cell population from canine kidney. Monolayers of these cells form domes, an indication of active ion transport, and this process is inhibited by ouabain. This technique allows the isolation of primary cultures of renal epithelial cells, free of fibroblasts, for the characterization of biochemical and physiological properties related to renal function.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jefferson, D M -- Cobb, M H -- Gennaro, J F Jr -- Scott, W N -- New York, N.Y. -- Science. 1980 Nov 21;210(4472):912-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7434005" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biological Transport, Active ; Cell Adhesion ; Cells, Cultured ; Culture Media ; Dogs ; Epithelium/metabolism ; Female ; Kidney/*cytology ; Male ; Sodium/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    (-)Pentobarbital opens ion channels of long duration in cultured mouse spinal neurons (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-07-25
    Description: Intracellular recordings from voltage-clamped mouse spinal neurons in tissue culture were used to study the membrane mechanisms underlying inhibitory responses to gamma-aminobutyric acid and the (-) isomer of pentobarbital. Fluctuation analysis suggested that both substances activated ion channels in the membranes. However, the channels activated by pentobarbital remained open five times longer than those activated by gamma-aminobutyric acid.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mathers, D A -- Barker, J L -- New York, N.Y. -- Science. 1980 Jul 25;209(4455):507-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6248961" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Membrane/drug effects/physiology ; Cells, Cultured ; Ion Channels/drug effects/*physiology ; Membrane Potentials/drug effects ; Mice ; Neurons/drug effects/*physiology ; Pentobarbital/*pharmacology ; Spinal Cord/*physiology ; gamma-Aminobutyric Acid/pharmacology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    Isolation of mutants of an animal virus in bacteria (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-09-19
    Description: Mutants of animal viruses can be isolated in bacteria by recombinant DNA methods. Since no viral functions are required for propagation of recombinants in bacteria, viral mutants with lethal changes in cis- or trans-acting elements can be isolated, as well as partially or conditionally defective mutants. In the cases of viruses with small DNA genomes, such as the tumorigenic simian virus 40 (SV40), the entire viral DNA can be inserted into the bacterial plasmid pBR322 and cloned in Escherichia coli. Recombinant plasmids with a single copy of SV40 DNA cause morphological transformation of mouse cells in culture with the same efficiency as SV40 DNA isolated from virus-infected monkey cells, but the recombinant DNA is noninfectious and replicates poorly in permissive cells. However, SV40 DNA excised from the plasmid replicates as well as authentic viral DNA and is fully infectious. SV40 mutants with small deletions or base substitutions have been isolated by in vitro site-specific or random local mutagenesis of recombinant DNA followed by cloning in E. coli. Many of the mutants thus isolated are defective in specific viral functions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Peden, K W -- Pipas, J M -- Pearson-White, S -- Nathans, D -- New York, N.Y. -- Science. 1980 Sep 19;209(4463):1392-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6251547" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antigens, Neoplasm/*genetics ; Antigens, Viral/genetics ; Cell Transformation, Viral ; Cells, Cultured ; Chromosome Deletion ; DNA, Recombinant ; DNA, Viral/*genetics ; Escherichia coli ; *Mutation ; Simian virus 40/*genetics ; Viral Proteins/*genetics ; Virus Replication
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    "Transdifferentiation" of C6 glial cells in culture (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-04-11
    Description: The activities of cyclic nucleotide phosphohydrolase, an enzyme marker for oligodendrocytes, and glutamine synthetase, an enzyme marker for astrocytes, were studied at early (21 to 26) and late (82 to 88) cell passages. The activity of cyclic nucleotide phosphohydrolase was markedly high and that of glutamine synthetase was low in the early passages, but this relation was reversed in the late passages. These findings suggest a "transdifferentiation" of C6 glial cells with passage in culture.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Parker, K K -- Norenberg, M D -- Vernadakis, A -- New York, N.Y. -- Science. 1980 Apr 11;208(4440):179-81.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6102413" target="_blank"〉PubMed〈/a〉
    Keywords: 2',3'-Cyclic-Nucleotide Phosphodiesterases/metabolism ; Animals ; Astrocytes/enzymology ; *Cell Differentiation ; Cells, Cultured ; Glutamate-Ammonia Ligase/metabolism ; Neuroglia/*enzymology ; Oligodendroglia/enzymology ; Rats
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
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    Cells isolated from the embryonic neural retina differ in behavior in vitro and membrane structure (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-08-29
    Description: Several subpopulations of cells were isolated from trypsin-dissociated embryonic (14 days) chick retinas. The cells of each subpopulation differed in associative behavior measured by cell aggregation and stationary culture assays and in glycoproteins that contain glucosamine. Freeze-fracture analysis showed that these populations also differed in intramembrane particle content.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sheffield, J B -- Pressman, D -- Lynch, M -- New York, N.Y. -- Science. 1980 Aug 29;209(4460):1043-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7403867" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Adhesion ; Cell Fractionation/methods ; Cell Membrane/ultrastructure ; Cells, Cultured ; Chick Embryo ; Membrane Proteins/metabolism ; Retina/cytology/*embryology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
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    Human rotavirus type 2: cultivation in vitro (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-01-11
    Description: A strain of type 2 human rotavirus (Wa) was grown to relatively high titer through 14 passages in primary cultures of African green monkey kidney (AGMK) cells. This passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. In contrast, virus present in the stool of patient Wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in African green monkey kidney cells. Prior to each passage in cell culture, the virus was treated with trypsin and the inoculated cultures were centrifuged at low speed. Cultivation of a type 2 human rotavirus should aid attempts to characterize this virus and to develop a means of immunoprophylaxis for a serious diarrheal disease of human infants.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wyatt, R G -- James, W D -- Bohl, E H -- Theil, K W -- Saif, L J -- Kalica, A R -- Greenberg, H B -- Kapikian, A Z -- Chanock, R M -- New York, N.Y. -- Science. 1980 Jan 11;207(4427):189-91.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6243190" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antigens, Viral/analysis ; Cells, Cultured ; Diarrhea, Infantile/microbiology ; Germ-Free Life ; Haplorhini ; Humans ; Infant ; RNA Viruses/*growth & development ; Rotavirus/*growth & development/immunology ; Swine
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
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    Malignant potential of murine stromal cells after transplantation of human tumors into nude mice (1981)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1981-04-03
    Description: Human malignant cancer tumors grafted into nude mice produce tumors containing both human cancer cells and the host's stromal cells. After short-term propagation of these tumors in vitro, the murine mesenchymal cells appear transformed and are tumorigenic in nude mice. However, established human cancer cell lines fail to similarly after adjacent murine stromal cells when used to produce tumors in nude mice. These experiments suggest that cancer cells may recruit normal cells to become malignant, qualifying the view of the clonal (unicellular) origin of cancer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Goldenberg, D M -- Pavia, R A -- 1R01 CA17198/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1981 Apr 3;212(4490):65-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7209521" target="_blank"〉PubMed〈/a〉
    Keywords: Adenocarcinoma/pathology ; Animals ; Cell Line ; Cells, Cultured ; Colonic Neoplasms/pathology ; Fibrosarcoma/*etiology ; Humans ; Karyotyping ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Middle Aged ; Neoplasm Transplantation ; Neoplasms, Experimental/*etiology ; Transplantation, Heterologous
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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