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  • 1
    Electronic Resource
    Electronic Resource
    Physiological aspects of genome variability in tissue culture. I. Growth phase-dependent differential DNA methylation of the carrot genome (Daucus carota L.) during primary culture (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 809-815 
    Details
    ISSN: 1432-2242
    Keywords: Daucus carota L. ; Tissue culture ; Differential DNA methylation ; Cell-division growth ; Kinetin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Investigations were performed on growth phase-dependent EcoRII site-specific DNA methylation of the carrot genome during primary culture to elucidate physiological aspects of genome DNA variability in tissue culture. While DNA methylation of the root cambium and the secondary phloem and petioles of carrot leaves were strikingly different, the methylation level of the secondary phloem seemed to be independent of cultivar origin, the age of the plants and the extent of secondary root growth. As was shown earlier a change in the differentiated state of the secondary phloem by tissue culture leads to changes in genome modification. Whereas de novo methylation was observed during the first 2 weeks of growth initiation, the results presented demonstrate genome de-methylation during the transition to stationary growth indicating differential εnome methylation during different phases of culture. The presence of kinetin in the nutrient medium of the primary culture was found to be antagonistic to changes in genome modification in general. De novo methylation and subsequent de-methylation of the carrot genome are discussed as gross changes obviously essential to molecular genome differentiation during tissue culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220964
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  • 2
    Electronic Resource
    Electronic Resource
    Physiological aspects of genome variability in tissue culture. II. Growth phase-dependent quantitative variability of repetitive BstNI fragments of primary cultures of Daucus carota L. (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 816-823 
    Details
    ISSN: 1432-2242
    Keywords: Daucus carota L. ; Tissue culture ; Differential DNA replication ; Cell-division growth ; Cell determination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Systematic investigations on the occurrence of differential DNA replication in carrot cultures, expressed at the total genome level, were performed. The genome of Daucus carota L. could be characterized by a pattern of repetitive BstNI fragments that was independent of tissue specificity or cultivar differences. Characterization of the genomic DNA of the secondary phloem of carrot roots, in comparison to the DNA of the induced primary cultures at different growth phases, revealed dramatic differences in the copy number of the repetitive fragments. Highly proliferative tissue showed extensive reduction in the proportion of repetitive sequences in the genome in all of the 37 investigated variants. In contrast, during subsequent transition to stationary growth the repetitive fragments re-amplified. The results suggest that the quantitative genome organisation was involved in the regulation of the growth potential of cells. A hypothesis is discussed suggesting a determining influence of the observed differential DNA replication on cell-cycle rates and the cell program of proliferative tissue by structural and positioning effects on DNA loops. To study the causality of somaclonal variation, research on the relationship between physiological genome variability and the induction of heritable changes is recommended.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220965
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    Paper (German National Licenses)
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