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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 109 (1976), S. 31-35 
    ISSN: 1432-072X
    Keywords: Scanning electron microscopy ; Chlamydomonas ; Cell agglutination ; Cell fusion ; Flagella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A technique has been developed by which mating gametes of Chlamydomonas eugametos can be studied in the Scanning Electron Microscope. A detailed description of the mating process, from the initial flagellar agglutination until the release of free vis-à-vis pairs, is presented. Flagella appear to agglutinate at random points on their surface. This is followed by a rapid increase of the contact area such that they “line-up” tip to tip. Flagella always exhibit a typical position prior to cell fusion. After cell fusion the flagella of a pair separate rapidly while the female have shortened about 33%. In a vis-à-vis pair the plasma bridge has contracted. The observations are interpreted in terms of a specific reorganization of the sexuale aggregate.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 117 (1978), S. 131-134 
    ISSN: 1432-072X
    Keywords: Chlamydomonas ; Cell fusion ; Inhibitor ; Plasma papilla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By a short treatment with acid of mt - gametes of Chlamydomonas eugametos, a factor is released which prevents gametic cell fusion, without affecting the viability of the cells. It has a very rapid action. By means of scanning electron microscopy it is shown that the factor has no influence on flagellar adhesion nor on the formation of a plasma papilla by cells of either mating type, but that it specifically inhibits the fusion of these papillae. Evidence is presented suggesting that this inhibitor has a predominant effect on mt + gametes. In cell pairs which are blocked with respect to papillar fusion, no flagellar disengagement occurs, which indicates that loss of agglutinability is a direct consequence of cell fusion.
    Type of Medium: Electronic Resource
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