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  • Cell fusion  (1)
  • DNA gyrase  (1)
  • Key words Dictyostelium discoideum   (1)
  • 1
    Electronic Resource
    Electronic Resource
    Induction of cell fusion by a factor released by the cellular slime mold Polysphondylium pallidum (1991)
    Springer
    Archives of microbiology 156 (1991), S. 159-162 
    Details
    ISSN: 1432-072X
    Keywords: Cell fusion ; Cell fusion inducing factor ; Cellular slime mold ; Polysphondylium pallidum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heterokaryons and hybrid cells, which are extremely useful for research in cell biology, can be produced artificially by treating cells with either polyethylene glycol or certain inactivated viruses that alter the plasma membrane. We report here a novel cell-fusion inducing factor secreted by CK-8 strain cells of cellular slime mold Polysphondylium pallidum. Treatment of other strains or other species of cellular slime molds, such as NC-4 of Dictyostelium discoideum with the diluted fraction, containing molecules larger than 50 kDa, of the conditioned medium of CK-8 cell culture induces cell fusion at high frequency and produces multinucleated large cells. This cell fusion is inducible between cells of either a single strain or of two different strains of cellular slime molds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249108
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  • 2
    Electronic Resource
    Electronic Resource
    Group-I introns in the cytochrome c oxidase genes of Dictyostelium discoideum : two related ORFs in one loop of a group-I intron, a cox1/2 hybrid gene and an unusually large cox3 gene (1997)
    Springer
    Current genetics 31 (1997), S. 80-88 
    Details
    ISSN: 1432-0983
    Keywords: Key words Dictyostelium discoideum  ;  Mitochondrial DNA  ;  Group-I intron  ;  Cytochrome oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The DNA sequences of cytochrome oxidase (subunits 1, 2 and 3) genes of the cellular slime mold Dictyostelium discoideum mitochondria were determined. The genes for subunits 1 and 2 have a single continuous ORF (COX1/2) which contains four group-I introns. The insertion sites of the two group-I introns (DdOX1/2.2 and DdOX1/2.3) coincide with those of fungal and algal group-I introns, as well as a liverwort group-I intron, in the cytochrome oxidase subunit 1. Interestingly, intron DdOX1/2.2 has two free-standing ORFs in a loop (L8) which have similar amino-acid sequences and are homologous to ai4 DNA endonuclease (I-Sce II) and bi4 RNA maturase found in group-I introns of Saccharomyces cerevisiae mitochondrial DNA. Two group-I introns (DdOX1/2.3 and DdOX1/2.4) also have a free-standing ORF in loop 1 and loop 2, respectively. These results show that these group-I introns and the intronic ORFs have evolved from the same ancestral origin, but that these ORFs have been propagated independently.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050179
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  • 3
    Electronic Resource
    Electronic Resource
    Formation of deletion in Escherichia coli between direct repeats located in the long inverted repeats of a cellular slime mold plasmid: Participation of DNA gyrase (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 1-5 
    Details
    ISSN: 1617-4623
    Keywords: Escherichia coli ; Dictyostelium ; DNA gyrase ; Deletion ; Plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We constructed a recombinant plasmid containing the 2.1 kb HindIII fragment of plasmid pDG1, isolated from the cellular slime mold (Dictyostelium sp. strain GA11), and using pAG60 as cloning vector. We found that deletions of the recombinant plasmid took place frequently in Escherichia coli wild-type cells. However, the deletion was not observed when the plasmid was introduced into a strain that was an isogenic temperature-sensitive mutant of the gyrA gene. These results suggest that E. coli DNA gyrase is involved in the mechanisms of the deletion formation. It was shown that the 1.0 kb deletant derived from the 2.1 kb HindIII insert was produced by elimination of a 1.1 kb region. Sequence analysis of the deletants showed that cutting and rejoining took place between two out of the six nearly perfect direct repeats [21 bp palindromic sequences; AAAAAA(T/C)GGC(G/C)GCC(A/G)TTTTTT], located near the distal ends of the inverted repeats, preserving one copy of the repeats. These sequences consist of local short inverted repeats, where cutting and rejoining occur at one of the two regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340170
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