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  • Cell division abnormalities  (1)
  • Differentiation (moss)  (1)
  • 1
    ISSN: 1432-2048
    Keywords: Bryophyta ; Bud induction (mosses) ; Caulonema, specific proteins ; Cytokinin and bud induction (mosses) ; Differentiation (moss) ; Funaria ; Physcomitrella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The bud-inducing effect of the cytokinin N6-(Δ2-isopentenyl)-adenine (i6-Ade) was examined in the moss Physcomitrella patens growing in liquid culture. Under these conditions, buds could be induced on chloronemata as well as on caulonemata. By application of i6-Ade, bud-formation was accelerated in both types of tissue. The number of buds, their size and their site of development were dependent on the concentration of the cytokinin in the range of 10-7 M to 10-5 M. Moreover, the percentage of caulonema cells increased with a cytokinin concentration of 10-5 M. These results indicate that chloronema cells may also function as target cells for exogenous cytokinins. The composition of proteins from caulonemata and chloronemata of two different species (P. patens and Funaria hygrometrica), grown on solid medium were compared. No differences could be detected between the protein patterns of caulonemata and chloronemata of the same species while between the two species the differences were obvious.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: Physcomitrella protonemata ; Chloroplast division ; Cytoskeleton ; Cell division abnormalities ; Microinjection ; Moss mutant ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An X-ray induced mutant (PC22) of the moss,Physcomitrella patens was analysed with respect to its morphology, physiology and suitability for microculture techniques. The mutant protonemata are defective in bud formation and in chloroplast division. As a consequence of the latter, giant chloroplasts are formed which disturb the development of the phragmoplast, the formation of regular cross walls, and cell division. Abnormal cross walls are rich in callose. The actin cytoskeleton was found to be less regularly developed in the mutant than in the wild type. Three-dimensional analysis of the microtubular arrangement with confocal laser scan microscopy demonstrates a close association between spindle- or phragmoplast- and “interphase”-microtubules. The deficiencies in chloroplast division and in bud formation can partly be compensated for by exogeneously applied cytokinin. The suitability of this particular developmental mutant for further studies was shown by regeneration of protoplasts in microculture and microinjection of the fluorochrome Lucifer yellow into the chloroplast.
    Type of Medium: Electronic Resource
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