Publication Date:
2004-09-14
Description:
Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 and other guanosine triphosphatases, the subcellular location of activation is a critical determinant of cell behavior. However, current approaches are limited in their ability to examine the dynamics of Cdc42 activity in living cells. We report the development of a biosensor capable of visualizing the changing activation of endogenous, unlabeled Cdc42 in living cells. With the use of a dye that reports protein interactions, the biosensor revealed localized activation in the trans-Golgi apparatus, microtubule-dependent Cdc42 activation at the cell periphery, and activation kinetics precisely coordinated with cell extension and retraction.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nalbant, Perihan -- Hodgson, Louis -- Kraynov, Vadim -- Toutchkine, Alexei -- Hahn, Klaus M -- GM57464/GM/NIGMS NIH HHS/ -- GM64346/GM/NIGMS NIH HHS/ -- R01 GM057464/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Sep 10;305(5690):1615-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7365, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15361624" target="_blank"〉PubMed〈/a〉
Keywords:
Actins/metabolism
;
Algorithms
;
Animals
;
*Biosensing Techniques
;
Cell Adhesion
;
Cell Line
;
Cell Membrane/*metabolism
;
Cell Polarity
;
Cell Surface Extensions/metabolism/ultrastructure
;
Endothelial Cells/metabolism/ultrastructure
;
Fibroblasts
;
Fluorescence
;
Fluorescent Dyes/chemistry/metabolism
;
Green Fluorescent Proteins
;
Humans
;
Luminescent Proteins
;
Mice
;
Microtubules/metabolism
;
Neutrophil Activation
;
Neutrophils/*metabolism
;
Proteins/chemistry/metabolism
;
Pseudopodia/metabolism
;
Pyrimidinones/metabolism
;
Sensitivity and Specificity
;
Wiskott-Aldrich Syndrome Protein
;
cdc42 GTP-Binding Protein/*metabolism
;
rho GTP-Binding Proteins/metabolism
;
trans-Golgi Network/*metabolism/ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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