Publication Date:
1988-06-03
Description:
The human immunodeficiency virus type 1 (HIV-1) uses the CD4 protein as a receptor for infection of susceptible cells. A candidate structure for the HIV-1 binding site on the CD4 protein was identified by epitope mapping with a family of eight functionally distinct CD4-specific monoclonal antibodies in conjunction with a panel of large CD4-derived synthetic peptides. All of the seven epitopes that were located reside within two immunoglobulin-like disulfide loops situated between residues 1 and 168 of the CD4 protein. The CD4-specific monoclonal antibody OKT4A, a potent inhibitor of HIV-1 binding, recognized a site between residues 32 and 47 on the CD4 protein. By analogy to other members of the immunoglobulin superfamily of proteins, this particular region has been predicted to exist as a protruding loop. A synthetic analog of this loop (residues 25 to 58) showed a concentration-dependent inhibition of HIV-1-induced cell fusion. It is proposed that a loop extending from residues 37 to 53 of the CD4 protein is a binding site for the AIDS virus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jameson, B A -- Rao, P E -- Kong, L I -- Hahn, B H -- Shaw, G M -- Hood, L E -- Kent, S B -- AI25784/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1988 Jun 3;240(4857):1335-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Biology, California Institute of Technology, Pasadena 91125.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2453925" target="_blank"〉PubMed〈/a〉
Keywords:
Antibodies, Monoclonal/immunology
;
Binding, Competitive
;
Cell Fusion
;
Disulfides/metabolism
;
Epitopes/immunology
;
Fluorescent Antibody Technique
;
HIV/immunology/*metabolism
;
HIV Envelope Protein gp120
;
Humans
;
Immunoglobulins
;
Peptide Fragments/chemical synthesis/immunology
;
Protein Conformation
;
Radioimmunoassay
;
Receptors, HIV
;
Receptors, Virus/*immunology
;
Retroviridae Proteins/immunology
;
T-Lymphocytes, Helper-Inducer/immunology/*microbiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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