Publication Date:
2001-06-26
Description:
It is widely assumed that the vital processes of transcription and translation are spatially separated in eukaryotes and that no translation occurs in nuclei. We localized translation sites by incubating permeabilized mammalian cells with [3H]lysine or lysyl-transfer RNA tagged with biotin or BODIPY; although most nascent polypeptides were cytoplasmic, some were found in discrete nuclear sites known as transcription "factories." Some of this nuclear translation also depends on concurrent transcription by RNA polymerase II. This coupling is simply explained if nuclear ribosomes translate nascent transcripts as those transcripts emerge from still-engaged RNA polymerases, much as they do in bacteria.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Iborra, F J -- Jackson, D A -- Cook, P R -- New York, N.Y. -- Science. 2001 Aug 10;293(5532):1139-42. Epub 2001 Jun 21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11423616" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Autoradiography
;
Biotin/metabolism
;
Boron Compounds/metabolism
;
COS Cells
;
Cell Fractionation
;
Cell Membrane Permeability
;
Cell Nucleus/*genetics/metabolism
;
Cycloheximide/pharmacology
;
Cytoplasm/metabolism
;
Fluorescence
;
HeLa Cells
;
Humans
;
Immunohistochemistry
;
Mitochondria/metabolism
;
*Protein Biosynthesis
;
Protein Synthesis Inhibitors/pharmacology
;
Protein Transport
;
Proteins/metabolism
;
RNA Polymerase II/metabolism
;
RNA, Transfer, Amino Acyl/metabolism
;
Ribosomes/metabolism
;
*Transcription, Genetic
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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