Publication Date:
1990-01-12
Description:
Normal cells in culture invariably undergo senescence, whereby they cease proliferation after a finite number of doublings. Irreversible changes in gene expression occurred in senescent human fetal lung fibroblasts: a non-cell cycle-regulated mRNA was partially repressed; an unusual polyadenylated histone mRNA was expressed; although serum induced c-H-ras, c-myc, and ornithine decarboxylase mRNA normally, ornithine decarboxylase activity was deficient; and serum did not induce mRNA for a replication-dependent histone and for the c-fos proto-oncogene. The loss of c-fos inducibility was the result of a specific, transcriptional block. The results suggest that senescent fibroblasts were unable to proliferate because of, at least in part, selective repression of c-fos; moreover, the multiple changes in gene expression support the view that cellular senescence is a process of terminal differentiation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Seshadri, T -- Campisi, J -- AG07114/AG/NIA NIH HHS/ -- New York, N.Y. -- Science. 1990 Jan 12;247(4939):205-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Boston University Medical School, MA 02118.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2104680" target="_blank"〉PubMed〈/a〉
Keywords:
Blood
;
Cell Division
;
Cell Line
;
Cell Survival/physiology
;
Embryo, Mammalian
;
Fibroblasts/*cytology/metabolism
;
Gene Expression/*physiology
;
Humans
;
Ornithine Decarboxylase/genetics
;
Proto-Oncogene Proteins/*genetics
;
Proto-Oncogene Proteins c-fos
;
Proto-Oncogene Proteins c-myc
;
Proto-Oncogene Proteins p21(ras)
;
RNA, Messenger/biosynthesis/genetics
;
*Suppression, Genetic
;
*Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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