ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
More than 90% of serum-deprived (starved) AKR-2B mouse fibroblasts are stimulated to divide by the addition of platelet-derived growth factor (PDGF)-BB. In density-arrested (nonstarved) cells, PDGF-BB affords protection from cell death without stimulation of cell division. In both cultivation conditions the cells express similar amounts of PDGF β-receptors and the receptor kinase activity was identical as judged by its autophosphorylation capacity. Three signaling pathways were studied in detail: (1) Phospholipase C-γ (PLC-γ) and [Ca2+]i increase, (2) activation of the phosphatidylinositol-3 kinase (PI-3 kinase), and (3) activation of mitogen activated kinases I and II (MAP kinases I and II). There was no difference in starved or nonstarved cells regarding PLC-γ activation, increase of [Ca2+]i, and stimulation of PL-3 kinase activity. But most remarkably the activation of MAP-I was largely suppressed in nonstarved cells. The implications of these signaling pathways in cell protection or cell division are discussed. © 1994 Wiley-Liss, Inc.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1041610219
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