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  • Chemical Engineering  (450)
  • Cell & Developmental Biology  (323)
  • ASTRONOMY  (302)
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  • 1980-1984  (968)
  • 1955-1959  (172)
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  • 1
    Electronic Resource
    Electronic Resource
    Intracellular concentration of elements in normal and dystrophic skeletal muscles of the chicken (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 103 (1980), S. 193-200 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In this study, the intracellular concentrations of six elements (mmole/kg dry weight) were directly measured in the muscle fibers of pectoralis major muscles of eight week old, genetically dystrophic and normal chickens by the X-ray microanalysis technique. The extent of muscle degeneration was evaluated by morphometric measurements of muscle fiber diameter and other histological changes. A significant increase in the concentration of intracellular sodium and chlorine was evident in dystrophic muscles. The concentration of intracellular sodium was 127.0 ± 35.0 in the muscle fibers of dystrophic chicks compared to 65.7 ± 16.5 in normal controls. The concentration of chlorine was 90.5 ± 27.5 and 54.1 ± 5.5 in the muscle fibers of dystrophic and normal chicks respectively. The intracellular concentrations of potassium, magnesium, phosphorous, and sulfur remained unchanged in the dystrophic condition. Morphometric studies revealed that the dystrophic pectoralis muscles contain fewer but thicker fibers per unit area compared to normal pectoralis muscles. The importance of these findings are discussed in relation to the results of earlier investigations.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041030204
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  • 2
    Electronic Resource
    Electronic Resource
    Evidence that myosin light chain phosphorylation regulates contraction in the body wall muscles of the sea cucumber (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 307-315 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The Ca2+ activation mechanism of the longitudinal body wall muscles of Parastichopus californicus (sea cucumber) was studied using skinned muscle fiber bundles. Reversible phosphorylation of the myosin light chains correlated with Ca2+-activated tension and relaxation. Pretreatment of the skinned fibers with ATPγS and high Ca2+ (10-5M) resulted in irreversible thiophosphorylation of the myosin light chains and activation of a Ca2+ insensitive tension. In contrast, pretreatment with low Ca2+ (10-8M) and ATPγS results in no thiophosphorylation of the myosin light chains or irreversible activation of tension. These results are consistent with a Ca2+-sensitive myosin light chain kinase/phosphatase system being responsible for the activation of the muscle. Other agents known to have an effect upon the Ca2+-activated tension in skinned vertebrate smooth muscle fibers (trifluoperazine, catalytic subunit of the cyclic AMP-dependent protein kinase, and calmodulin) did not have an effect on myosin light chain phosphorylation or Ca2+-activated tension. These results suggest a different type of myosin light chain kinase than is found in vertebrate smooth muscle is responsible for the activation of parastichopus longitudinal body wall muscle.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041120302
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  • 3
    Electronic Resource
    Electronic Resource
    Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 339-351 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120100402
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  • 4
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    Evidence for material between Saturn's A and F rings from the Voyager 2 photopolarimeter experiment (1984)
    In:  Other Sources
    Details
    Publication Date: 2011-08-19
    Description: The region in the Saturn system between the F ring and the outer edge of the A ring is an area that appears, in images from the imaging experiment, to be virtually devoid of material except for three small satellites. Near the orbit of 1980S28, Atlas - the innermost satellite - the Voyager Photopolarimeter Stellar Occultation data show a discontinuity in count rate which marks a boundary between the tenuous material near the outer edge of the A ring and the orbit of Atlas. The data pertaining to this region have been examined with the aid of statistics and models generated from other similar ring structures. It is concluded that the discontinuity is real, implying the existence of tenuous material of normal optical depth of 0.01 to 0.006 in this region.
    Keywords: ASTRONOMY
    Type: Icarus (ISSN 0019-1035); 60; 409-415
    Format: text
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  • 5
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    High-resolution observations of the QSO 3C 345 at 1.3 centimeters (1981)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: High-resolution VLBI observations made at a frequency of 22.235 GHz of the quasar 3C 345 are discussed. Antennas located at the Crimean Astrophysical Observatory, USSR; Onsala, Sweden; Effelsberg, West Germany; and the Haystack Observatory, Massachusetts were employed at 4-min integration times to provide baselines ranging up to 5.5 x 10 to the 8th wavelengths. About 40% of the total flux density of 7.85 Jy, observed in November 1977, and 8.05 Jy, observed in October 1978, is found to originate in an unresolved component of the quasar core in a region less than 0.1 milliarcsec in diameter. The elongated jet-like component of the quasar is observed to contain several peaks of emission extending up to 6 milliarsec from the core which decreased in extent between the two observations.
    Keywords: ASTRONOMY
    Type: Astrophysical Journal; vol. 243
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    IUE observations of dust albedo in nebulae (1982)
    In:  Other Sources
    Details
    Publication Date: 2011-08-18
    Description: The wavelength dependence of the effective albedo of the dust at several positions near the Hourglass region of M8 was determined. Accurate estimates of the contribution of the continuous gas emission can be made using the region 1400A to 1600A. The main uncertainty results from the peculiar extinction of the exciting star, Herschel 36, and the possibility that extinction between the star and the reflecting portions of the nebula exists and needs to be corrected for. The albedo for some planetaries such as NGC 6543 were also investigated. The main uncertainty here is estimating instrumental contributions to the scattering in observing these small objects.
    Keywords: ASTRONOMY
    Type: NASA. Goddard Space Flight Center Advan. in Ultraviolet Astron.; p 405-408
    Format: text
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  • 7
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    Centaurus A /NGC 5128/ at 2 keV-2.3 MeV - HEAO 1 observations and implications (1981)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: The active-nucleus galaxy Centaurus A has been studied at 2 keV-2.3 MeV using data from the UCSD/MIT hard X-ray and low-energy gamma-ray instrument and the GSFC/CIT cosmic X-ray experiment on HEAO-1. It is found that an E exp -1.60 + or - 0.03 power law spectrum breaking to E exp -2.0 + or - 0.2 at 140 keV best describes the January and July 1978 data. The average intensity was 50% higher during the January observations. Upper limits to unresolved lines at 511 keV and 1.6 MeV were found to be 6.5 x 10 to the -4th photons/sq cm-s and 2.2 x 10 to the -4th photons/sq cm-s, respectively, at the 90% confidence level. The present data are consistent with the detailed calculations of the synchrotron self-Compton mechanism; they may also agree, marginally, with the predictions of emission from spherical accretion onto black holes.
    Keywords: ASTRONOMY
    Type: Astrophysical Journal; vol. 244
    Format: text
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    NASA TECHNICAL REPORTS
  • 8
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    Development of a radio-astrometric catalog by means of very long baseline interferometry observations (1981)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: The Jet Propulsion Laboratory has been developing a radio-astrometric catalogue for use in the application of radio interferometry to interplanetary navigation and geodesy. The catalogue consists of approximately 100 compact extragalactic radio sources whose relative positions have formal uncertainties of the order of 0.01 arcsec. The sources cover nearly all of the celestial sphere above -40 deg declination. By using the optical counterparts of many of these radio sources, this radio reference frame has been tied to the FK4 optical system with a global accuracy of approximately 0.1 arcsec. This paper describes the status of this work.
    Keywords: ASTRONOMY
    Type: Reference coordinate systems for earth dynamics; Sep 08, 1980 - Sep 12, 1980; Warsaw; Poland
    Format: text
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  • 9
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    High resolution large area modular array of reflectors /LAMAR/ Wolter type I X-ray telescope for Spacelab (1982)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: The Spacelab Wolter type I X-ray telescope, which is intended for both astronomical observations and the functional verification of the future Large Area Modular Array of Reflectors (LAMAR) concept, comprises five mirrors and is designed to have a blur circle radius of 20 arcsec, with effective areas of (1) 400 sq cm at 0.25 keV, (2) 200 sq cm in the 0.5-2.0 keV range, and (3) 50 sq cm between 2 and 5 keV. A rotary interchange mechanism allows either of two imaging proportional counters to be placed at the telescope focus. The telescope's primary objective is the observational study of galactic and extragalactic X-ray sources, extending the work of the Einstein Observatory to fainter sources and higher energies. Secondarily, the costs and performance to be expected from the use of this telescope type in the LAMAR mission will be assessed.
    Keywords: ASTRONOMY
    Type: NASA-ESA Spacelab systems and programs; Apr 23, 1981 - Apr 24, 1981; Washington, DC
    Format: text
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  • 10
    Electronic Resource
    Electronic Resource
    Intercellular bridges in ovaries of the newborn gerbil (1984)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 29-35 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This study describes intercellular bridges in the ovaries of neonatal gerbils. Electron microscopy has revealed the presence of true intercellular bridges, connecting oogonia or oocytes, in ovaries of newborn gerbils. The cytoplasm of the intercellular channels is similar to that of the connected cells, with mitochondria, smooth and rough endoplasmic reticulum, and free ribosomes present. Lysosomes are also occasionally present in the intercellular bridges and they may be involved in early waves of oocyte atresia. An electrondense substance, 350-500 Å thick, is located immediately beneath the unit membrane of the intercellular bridges. Accumulation of electron-dense material increases the thickness of the walls of the intercellular bridges, supporting and maintaining the patency of the channels. It is suggested that the intercellular channels probably allow the interchange of nutrients, organelles, and possibly regulatory materials as well.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051800105
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