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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of classes of compound of environmental concern: I. Nitroaromatic compounds (1991)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 14 (1991), S. 601-607 
    Details
    ISSN: 0935-6304
    Keywords: Nitroaromatic compounds ; Fused silica open tubular column ; Dual-column/dual-detector GC system ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Thirty five nitroaromatic compounds (currently listed in EPA Method 8091) and 1,4-naphthoquinone have been separated on DB-5-DB-1701 and SPB-5-SPB-1701 column pairs, which were in each case connected to an inlet splitter and separate electron capture detectors. Retention times are included for 21 additional compounds evaluated for their suitability as internal standards and/or surrogate compounds for incorporation into Method 8091. Method reproducibility and linearity are discussed, and results are presented for extracts of two real samples spiked with the 35 nitroaromatic compounds and 1,4-naphthoquinone and analyzed using the dual-column-dual-detector arrangement.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240140909
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of an active GST-human Cdc2 fusion protein kinase expressed in the fission yeast Schizosaccharomyces pombe: A new approach to the study of cell cycle control proteins (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 1631-1638 
    Details
    ISSN: 0749-503X
    Keywords: Schizosaccharomyces pombe ; cell cycle ; Cdc2 kinase ; GST ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Characterization of cdk (cyclin dependent kinases) substrates and studies of their regulation require purified enzymatic complexes of cdc2-related catalytic and cyclin regulatory subunits. We produced human Cdc2 kinase in the fission yeast Schizosaccharomyces pombe as a fusion protein with glutathione S-transferase (GST). The GST-human Cdc2p fusion protein was active in vivo since it rescued a temperature-sensitive allele of cdc2. The fusion protein was purified using a one-step chromatography procedure with glutathione-Sepharose and exhibited a catalytic activity in vitro. Yeast cyclin B and suc1 were found in association with GST-Cdc2. A 17-fold stimulation of GST-Cdc2 kinase activity was obtained by incubation of recombinant human cyclin A with the S. pombe cellular extract prior to affinity purification. This indicates that cyclin concentration is limiting in this overexpression system. These findings describe a fast and easy production of active recombinant human Cdc2 kinase in yeast that can be used for biochemical studies.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320101212
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