ISSN:
1432-203X
Keywords:
Key wordsTypha latifolia
;
Cattail
;
Remediation
;
Shoot regeneration
;
Wetland monocot
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A regeneration system for broadleaf cattail (Typha latifolia), a common freshwater wetland monocot, was established. Callus was induced from 3 day-old seedlings on Murashige and Skoog medium supplemented with picloram or 2,4-dichlorophenoxyacetic acid (2,4-D). Picloram induced more callus growth than 2,4-D. Callus proliferated predominantly from the root-shoot junction and sporadically from the roots. Upon transfer to N6-benzyladenine-supplemented medium, callus regenerated shoots. The mode of regeneration was organogenesis, with shoots arising from organized areas of callus. The regenerated shoots rooted in vitro and, on transfer to pots containing a commercial potting mix, were established in a greenhouse. The plants grew vigorously and produced a high root mass. No albinos and no morphological aberrations were observed in the regenerates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050534
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