ISSN:
0935-6304
Keywords:
Gas chromatography
;
Capillary, glass
;
Gas chromatographic profiles
;
n-Butylboronates
;
Alkyl-oximes
;
Primary prostaglandins
;
6-Keto prostaglandin F1α
;
Thromboxane B2
;
Isomers of prostaglandin F1α
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
This work describes the use of glass capillary columns (GCC) in the rapid concurrent analysis of primary prostaglandins (PGs) (e. g. PGE2, PGE2, PGF2α) and other functionally significant metabolites of arachidonic acid (AA) such as TXB2 and 6-keto PGF1α. The use of a new multistep mixed derivatization approach that generates the methyl esters of n-butylboronate, pentafluorobenzyloxime, trimethylsilyl ether derivatives of these compounds remarkably simplifies the GC profiling of the three main pathways of AA metabolism (PGs, thromboxane, and prostacyclin). Furthermore, isomeric species giving very similar or identical mass spectral patterns can be easily identified by their relative retention times on high efficiency capillary columns.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240040903
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