ISSN:
1432-0983
Keywords:
Candida
;
Peroxisomes
;
Hydratase-dehydrogenase-epimerase
;
Import
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We present a system for studying peroxisomal protein targeting in Candida. We have expressed the Candida tropicalis gene encoding hydratase-dehydrogenase-epimerase (HDE) in Candida albicans. Immunoblot analyses of C. albicans transformants demonstrate the presence of oleic-acid inducible HDE (100 kDa) in peroxisomes of transformed cells, but not of control cells. Peroxisomes isolated from transformed cells show increased β-hydroxyacyl-CoA dehydrogenase specific activity, indicating that HDE is imported into peroxisomes of C. albicans where it is enzymatically active. C. albicans provides a useful model for the study of protein targeting to peroxisomes in vivo.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00313075
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