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  • 1
    Electronic Resource
    Electronic Resource
    Microsatellite-based cancer detection using capillary array electrophoresis and energy-transfer fluorescent primers (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 1742-1749 
    Details
    ISSN: 0173-0835
    Keywords: Cancer diagnosis ; Short tandem repeat ; Capillary electrophoresis ; Energy-transfer primers ; Microsatellites ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The development of sensitive, rapid, and accurate methods and apparatus for high-throughput short tandem repeat (STR) analysis will be critical for the use of microsatellite alteration in cancer screening. Here we show that STR-based bladder cancer diagnosis can be performed using capillary array electrophoresis and two-color labeling with energy-transfer (ET) fluorescent primers. Rapid (≤35 min) separations are achieved on capillary arrays using replaceable separation matrices and the allelic ratios are quantitatively determined with a precision of ± 10%. With this precision, a variation of 20% was considered diagnostically significant. These methods provide a significant improvement in the speed, ease, and precision of STR analyses compared to slab gel electrophoresis.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150181007
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    Paper (German National Licenses)
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  • 2
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    Integration of a Micro-Chip Amino Acid Chirality Detector into the MOD Instrument Concept (2004)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: The MOD (Mars Organic Detector) instrument concept consists of a sublimation apparatus for organic compound isolation connected to a microfabricated microfluidic analyzer containing a sipper, pumps and a separation channel for organic compound characterization. The target organic compounds are amino acids and polycyclic aromatic hydrocarbons (PAHs). Solid samples are placed within the sublimation apparatus and heated to release organic compounds which sublime onto a cold finger. Half of the cold finger is coated with fluorescamine. which reacts with amino acids and other primary amines to generate an intense fluorescent derivative while the other half is uncoated and is used to directly detect PAH fluorescence, A capillary sipper is then used to dissolve and sample the labeled amino acids and integrated microfabricated pumps transport the labeled amino acids to the chip for analysis. The sample is separated using capillary zone electrophoresis (CZE) together with chiral dextrins to determine amino acid composition and chirality. During the grant period, the following steps have been completed toward the development of a robust instrument and chemistry.
    Keywords: Electronics and Electrical Engineering
    Format: application/pdf
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    NASA TECHNICAL REPORTS
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