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  • 1
    ISSN: 1432-0983
    Keywords: Laccas ; Phenol-oxidas ; Rhizoctonia solani ; Fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four distinct laccase genes,lcc1, lcc2, lcc3 andlcc4, have been identified in the fungusRhizoctonia solani. Both cDNA and genomic copies of these genes were isolated and characterized. Hybridization analyses indicate that each of the four laccase genes is present in a single copy in the genome. TheR. solani laccases can be divided into two groups based on their protein size, intron/exon organization, and transcriptional regulation. Three of these enzymes have been expressed in the fungusAspergillus oryzae. Two of the recombinant laccases, r-lccl and r-lcc4, as well as the native lcc4 enzyme were purified and characterized. The purified proteins are homodimeric, comprised of two subunits of approximately 66 kDa for lcc4 and 50–100 kDa for the recombinant lccl protein. These laccases have spectral properties that are consistent with other blue copper proteins. With syringaldazine as a substrate, lcc4 has optimal activity at pH 7, whereas lcc1 has optimal activity at pH 6.
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  • 2
    ISSN: 1432-0983
    Keywords: Key words Laccas ; Phenol-oxidas ; Rhizoctonia solani ; Fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Four distinct laccase genes, lcc1, lcc2, lcc3 and lcc4, have been identified in the fungus Rhizoctonia solani. Both cDNA and genomic copies of these genes were isolated and characterized. Hybridization analyses indicate that each of the four laccase genes is present in a single copy in the genome. The R. solani laccases can be divided into two groups based on their protein size, intron/exon organization, and transcriptional regulation. Three of these enzymes have been expressed in the fungus Aspergillus oryzae. Two of the recombinant laccases, r-lcc1 and r-lcc4, as well as the native lcc4 enzyme were purified and characterized. The purified proteins are homodimeric, comprised of two subunits of approximately 66 kDa for lcc4 and 50–100 kDa for the recombinant lcc1 protein. These laccases have spectral properties that are consistent with other blue copper proteins. With syringaldazine as a substrate, lcc4 has optimal activity at pH 7, whereas lcc1 has optimal activity at pH 6.
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  • 3
    ISSN: 1573-2932
    Keywords: California ; acidic deposition ; human health ; ecological effects ; deposition fluxes ; material damage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract California's 14-year, $25 million acidic deposition program has studied the causes and effects of acidic air pollutants. In contrast to the eastern United States where sulfur-derived (S-derived) by-products from coal combustion dominate precipitation chemistry, nitrogen-derived (N-derived) acids predominate in wet and dry deposition in California. Adverse effects on the human lung have not been observed after short-term exposures to acidity, but extended exposures to ambient acidity may pose a chronic risk. No irreversible, adverse effects on surface waters in the Sierra Nevada mountain range or to the state's forests have been found due to extant acidic inputs. The longer-term outlook for forests is less certain because the impacts observed elsewhere occurred after decades of S and N deposition, but at lower ambient ozone levels. Ozone is the major air pollutant stressor for forests, but atmospheric N has the potential to cause adverse changes in soil nutrient cycling. Impacts on man-made materials in southern California (e.g., galvanized steel) were found to be minor. While California does not have an ambient air quality standard for acidic air pollutants, emissions of precursors have declined since the 1960's due to changes in industrial practices, improvements in technology, and adoption of control measures for ozone. Lowering emissions from motor vehicles will be emphasized to prevent future increases in N deposition.
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