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  • Calcium  (1)
  • Springer  (1)
  • Springer Nature
  • ZBW - Deutsche Zentralbibliothek für Wirtschaftswissenschaften, Leibniz-Informationszentrum Wirtschaft Kiel, Hamburg
  • Wiley
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  • Springer  (1)
  • Springer Nature
  • ZBW - Deutsche Zentralbibliothek für Wirtschaftswissenschaften, Leibniz-Informationszentrum Wirtschaft Kiel, Hamburg
  • Wiley
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    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 120 (1984), S. 209-215 
    ISSN: 1615-6102
    Keywords: Fusion ; Calcium ; Protoplast ; Membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rather than selecting for a chemical fusogen one can select for a fusogenic plant membrane (i.e., one that will fuse readily). Wild carrot suspension culture cells can be grown under conditions which cause the released protoplasts to have a high potential to fuse. Protoplast fusion is enhanced by calcium and inhibited by EGTA. When 10mM calcium (pH6.0) is added, fusion percentages of 60% are common. The mild fusion treatment appears to have no effect on callus regeneration and differentiation.
    Type of Medium: Electronic Resource
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