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Biochemical and physicochemical properties of the solubilized S-100 protein binding activity of synaptosomal particulate fractions (1983)

Donato, Rosario

Springer

Cellular and molecular neurobiology 3 (1983), S. 239-254

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ISSN: 1573-6830

Keywords: S-100 protein ; synaptosomes ; S-100 binding sites ; Ca2+-binding protein

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary 1. The125I-labeled S-100 specific binding to a Triton X-100 (TX-100) extract of synaptosomal particulate fractions (SYN) was investigated. 2. The results indicate that (a) S-100 binding to the TX-100 extract is partially irreversible after a critical association time at 37°C, while it is fully reversible after any association time at 4°C; (b) trypsin and phospholipase C partially reverse the S-100 binding, while phospholipase D enhances the interaction to some extent, in a dose-dependent way; (c) EDTA and high concentrations of NaC1 or KC1 are more efficient as inhibitors of the S-100 binding to the TX-100 extract than as125I-labeled S-100 dissociating agents, in analogy with previous observations with SYN; and (d) two main populations of solubilized S-100 binding sites can be evidenced by gel filtration and sucrose gradient centrifugation when low amounts of the TX-100 extract are processed and/or low S-100 concentrations are used, while two additional molecular species are separated when greater amounts of either factors are tested. 3. These results suggest the possibility that S-100 may be involved in the regulation of some membrane activities.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00710950

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Vibrational spectra and assignments of ethylene-diamine and its deuterated derivatives (1983)

Giorgini, Maria Grazia ; Pelletti, Maria Rosaria ; Paliani, Giulio ; [et al.]

Chichester [u.a.] : Wiley-Blackwell

Journal of Raman Spectroscopy 14 (1983), S. 16-21

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ISSN: 0377-0486

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Notes: The infrared and Raman spectra of ethylenediamine and its deuterated derivatives have been studied in both the liquid and solid states. An assignment of the fundamental vibrational modes for the four molecules is proposed assuming for the liquids a gauche configuration (C2 symmetry); the assignment is based on depolarization values, isotopic shifts and classic group frequencies correlations.

Additional Material: 2 Ill.