Publikationsdatum:
2022-05-26
Beschreibung:
Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B. V. for personal use, not for redistribution. The definitive version was published in Biochemical and Biophysical Research Communications 331 (2005): 1016-1024, doi:10.1016/j.bbrc.2005.03.231.
Beschreibung:
Two novel CYP1 genes from teleost fish constituting a new subfamily have been cloned.
These paralogous sequences are designated CYP1C1 and CYP1C2. Both genes were
initially obtained from untreated scup Stenotomus chrysops tissues by RT-PCR and
RACE. Scup CYP1C1 and CYP1C2 code for 524 and 525 amino acids, respectively, and
share 80-81% identity at the nucleotide and amino acid levels. Orthologues of CYP1C1
and CYP1C2 were identified in genome databases for other fish species, and both
CYP1B1 and CYP1C1 were cloned from zebrafish (Danio rerio). Phylogenetic analysis
shows that CYP1Cs and CYP1Bs constitute a sister clade to the CYP1As. Analysis of
sequence domains likely to have functional significance suggests the two CYP1Cs in
scup may have catalytic functions and/or substrate specificity that differ from each other
and from those of mammalian CYP1Bs or CYP1As. RT-PCR results indicate that
CYP1C1 and CYP1C2 are variously expressed in several scup organs.
Beschreibung:
This work was supported by EPA grant R 827102-01-0 and NIH grants 5 P42-ES07381
and ES04696. JVG is supported by a Ruth L. Kirschstein NRSA Fellowship (F32
ES012794).
Schlagwort(e):
Cytochrome P450
;
CYP1A
;
CYP1B1
;
CYP1C
;
PAH
;
Planar aromatic hydrocarbons
Repository-Name:
Woods Hole Open Access Server
Materialart:
Preprint
Format:
96256 bytes
Format:
288797 bytes
Format:
application/msword
Format:
application/pdf
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